TABLE 2 

VirCapSeq-VERT provides greater genome coverage and sequencing depth than HTS

LibrarybVirusLoad (copies)aGenome length (nt)No. of mapped positions% sequence mappedCoverageNo. of unmapped regionsUnmapped region length (nt)
MinMaxAvg
Pool 1a (lung, HTS)EV-D681057,3417,26899.0102,384932473
MERS-CoV10430,1131,8246.06020.11928,289
FLUAV -11042,3162,00586.57092.55311
FLUAV -22,3042,24897.570196.4256
FLUAV -32,2081,99890.490293.84210
FLUAV -41,7371,64294.530328.0295
FLUAV -51,5401,49497.010144.1346
FLUAV -61,4421,33492.510114.23108
FLUAV -71,00294894.610113.7254
FLUAV -886580192.600113.8365
           
Pool 1b (lung, VirCapSeq-VERT)EV-D681057,3417,341100.0038,0807,00500
MERS-CoV10430,11329,02096.37012113231,093
FLUAV -11042,3162,316100.005908,0615,23000
FLUAV -22,3042,304100.005698,0487,60800
FLUAV -32,2082,208100.008188,0404,84700
FLUAV -41,7371,737100.003238,0387,44900
FLUAV -51,5401,540100.009098,0037,09100
FLUAV -61,4421,442100.003487,9996,97500
FLUAV -71,0021,002100.00608,0566,21600
FLUAV -8865865100.004488,0065,76100
           
Pool 2a (blood, HTS)HHV-1105152,151151,97099.8804181424183
DENV-310510,70710,68799.8101,242622120
WNV10410,9455004.57010.1610,445
EBOV10318,9594,71624.87020.34314,243
CVV-S10490581890.39073.1387
CVV-M4,3052,63361.16051.1151,672
CVV-L6,8402,30933.79050.5174,531
           
Pool 2b (blood, VirCapSeq-VERT)HHV-1105152,151152,13399.9908,0015,373118
DENV-310510,70710,68899.8208,0687,774119
WNV10410,94510,42895.280214661517
EBOV10318,95916,41386.57039456112,546
CVV-S10490590499.8907,3192,30211
CVV-M4,3054,305100.0021,55140100
CVV-L6,8406,840100.0018588800
  • a Determined by qPCR of double-stranded-cDNA/DNA used for sequence library construction.

  • b See Table 1 for pool composition.