TABLE 1 

Dissociation constants of WT RctB and RctB ΔC159 for various DNA fragments

DNA probeAvg Kd (nM) ± SDa of:
WT RctBRctB ΔC159
12×6 Dam+0.0227 ± 0.01980.0094 ± 0.004
12×6 DamNDb13.84 ± 3.86
Inc 11197.6 ± 252.30.061 ± 0.11
Inc 122.98 ± 0.700.00016 ± 0.00025
11×68.50 ± 6.925.45 ± 2.87
11+1×67.85 ± 8.135.35 ± 6.25
Rev12×60.22 ± 0.160.29 ± 0.20
12×210.16 ± 7.140.26 ± 0.029
12×40.023 ± 0.00710.036 ± 0.029
12-10.036 ± 0.0260.0081 ± 0.0025
12-20.092 ± 0.110.012 ± 0.06
12-30.06 ± 0.0150.032 ± 0.0025
12-40.11 ± 0.0210.04 ± 0.029
12-50.087 ± 0.030.038 ± 0.025
12-60.093 ± 0.0130.17 ± 0.033
12-1351.07 ± 0.230.35 ± 0.59
12-2462.13 ± 0.401.20 ± 0.96
12-1245642.62 ± 22.235.66 ± 2.97
  • a Average values and standard deviations were obtained by quantitation of at least three independent binding experiments.

  • b ND, not determined.