U.S. porcine orthoreovirus strains show altered UTRs

SegmentSize (bp)5′ endORF/protein3′ end
Terminal sequenceaUTR (bp)RegionSize (aa)ClassProtein functionbUTR (bp)Terminal sequencea
L13,854GCUACA1819–38221,267λ3RNA-dependent RNA polymerase32ACUCAUC
L23,915GCUAUU1213–38821,289λ2Guanyltransferase, methyltransferase33AUUCAUC
L33,901GCUAAU1314–38411,275λ1RNA binding, NTPase, helicase, RNA triphosphatase60AUUCAUC
M12,304GCUAUU1314–2224736μ2Binds RNA NTPase80CUUCAUC
M22,205UGCUAAU3031–2157708μ1Cell penetration, transcriptase activation48AUCAUCA
S11,416UGCUAUU1415–1382, 73–435455, 120σ1, σ1sCell attachment34CACUUAA
S21,331GCUAUU1819–1275418σ2Binds dsRNA56ACUGACC
S31,198GCUAAA2728–1128366σNSInclusion formation, binds ssRNA70AAUCAUC
S41,196GCUAUU3132–1129365σ3, σ3a, σ3bBinds dsRNA67AUUCAUC
  • a The 5′ and 3′ untranslated regions (UTRs) of U.S. porcine strains FS03 and BM100 show mutations on the M2, S1, and S2 segments. The conserved terminal sequences are shown in boldface, and mutations are italicized.

  • b Predicted functions of different proteins encoded by the 10 segments analogous to known members of the Orthoreovirus genus are also indicated.