TABLE 2 

Indel differences between the initial (MSHR1043) and 139-month (MSHR6686) B. pseudomallei isolates from P314

Location in
MSHR1043
Nucleotide changeAffected gene
in MSHR1043
(K96243)e
Indel effect(s)aMutated in
MSHR1655?
Affected proteinPutative function(s)
of protein
seq0001
105149
CT→CNAdNANobNAUnknown
seq0001
416897
CCGCGACGCCGAG→CD512_01975
(BPSL0194)
Deletion (LGVA),
codon 20; neutral
NoHypothetical proteinUnknown
seq0001
1496938
GGC→GD512_06755
(BPSL1120)
Frameshift, codon 64;
premature stop
(381-103 aa); deleterious
NocLPS heptosyltransferase IBiosynthesis, modification
of LPS core
seq0003
131174
CT→CD512_07898
(BPSL1343)
Frameshift, codon 25;
premature stop
(270-110 aa); deleterious
YesHypothetical proteinUnknown
seq0003
388954
G→GGAD512_08953
(BPSL1936)
Frameshift, codon 100;
premature stop
(394-110 aa); deleterious
YesOacABiosynthesis, modification
of LPS O antigen
seq0003
537757
AC→ANot assigned
(BPSL1804)
Frameshift, codon 247 in
MSHR1043; increased length
(399–419 aa); deleterious
NobAmrAAntibiotic resistance
seq0003
721338
CGCCGGGGCGG→CD512_10308
(BPSL1687)
Frameshift, codon 85;
premature stop
(213-179 aa); deleterious
YesHypothetical proteinUnknown
seq0003
1076302
GC→GD512_11953
(BPSL2093)
Frameshift, codon 43;
premature stop
(96-73 aa); deleterious
YesHypothetical proteinUnknown
seq0007
181244
C→CCACTCGD512_15706
(BPSL2660)
Insertion (HS), codon 174
(205–207 aa); neutral
YesUreENitrogen fixation
seq0007
194989
T→TCD512_15766
(BPSL2672)
Frameshift, codon 272;
premature stop
(637-550 aa); deleterious
YesWbiIBiosynthesis, modification
of LPS O antigen
seq0007
196460
G→GCD512_15771
(BPSL2673)
Frameshift, codon 121;
premature stop
(336-141 aa); deleterious
NoWbiHBiosynthesis, modification
of LPS O antigen
seq0020
30723
ACGCAT→ANANANobNAUnknown
seq0024
104413
GCATCGA→GNANANobNAUnknown
seq0028
195058
A→ACD512_25678
(BPSS1494)
Frameshift, codon 183;
increased length
(245-854 aa); deleterious
YesDNA-binding
response regulator
DNA transcription
regulation, virulence
  • a  According to SnpEff (66) and PROVEAN (67). aa, amino acids.

  • b  Indel identified in MSHR1043; neither any other previously sequenced B. pseudomallei strains nor the two latter P314 isolates, MSHR1655 and MSHR6686, share these indels.

  • c  MSHR1655 has an insertion of a repetitive AHL amino acid motif at codon 278 (total length, 384 amino acids).

  • d  NA, not applicable.

  • e  A 2-bp insertion at position 298 of oacA (D512_08953) and a 1-bp insertion at position 815 of wbiI (D512_15766) have been previously identified (25, 26).