TABLE 2 

Numbers of independent rpoB mutant isolates used in luciferase reporter gene assays, competitive fitness assays, and qPCR assaysa

rpoB mutation(s)No. of independent rpoB mutant isolates used inb:
Luciferase reporter gene assaysCompetitive fitnessassaysqPCR
Original resistance mutation
    Q518L221
    Q518R111
    D521G721
    D521N221
    H531L221
    H531R222
    H531Y321
    S536F111
    S579F222
Original + compensatory mutation
    H531Cc441
    Q518R + E528D221
    H531Y + N573S221
  • a Isolates with the original resistance mutations in rpoB were obtained from the fluctuation test, and mutants with compensatory mutations were isolated from the selection experiment.

  • b The numbers of rpoB mutant isolates used in competitive fitness assays form subsets of those tested in luciferase reporter gene assays. Likewise, the numbers of isolates assayed using qPCR are subsets of those tested in competitive fitness assays. Small variations were observed among individual rpoB mutant isolates with the same rpoB mutations in all experiments. Three biological replicates of each isolate were assayed (n = 3).

  • c Two H531C isolates were evolved from the H531R rpoB mutant. The other two H531C isolates were evolved from the H531Y rpoB mutant.