TABLE 6 

Plasmids used in this study

NameCharacteristicsOrigin
pUC57Delivery vectorGeneArt, Germany
pUG6Template for loxP-KanMX-loxP cassette52
pUG27Template for loxP-Sphis5-loxP cassette52
pUG73Template for loxP-KlLEU2-loxP cassette52
pRS426-TEF1Sequence template for 2µ67
pRS316Sequence template for CEN6 ARS468
pRS416Sequence template for URA3 (including promoter and terminator)68
pUDC140URA3 CEN6 ARS4 pTPI1-pdhA-tTEF1 pTDH3-pdhB-tCYC1 pADH1-aceF-tPGI1 pTEF1-lpd-tADH1
pPGK1-lplA-tPMA1 pPGI1-lplA2-tPYK1 pMB1-AmpR
This study
pUDC143URA3 CEN6 ARS4 pTPI1-pdhA-tTEF1 pTDH3-pdhB-tCYC1 pADH1-aceF-tPGI1 pTEF1-lpd-tADH1
pMB1-AmpR
This study
pUDE333URA3 2µ pTPI1-pdhA-tTEF1 pTDH3-pdhB-tCYC1 pADH1-aceF-tPGI1 pTEF1-lpd-tADH1 pPGK1-
lplA-tPMA1 pPGI1-lplA2-tPYK1 pMB1-AmpR
This study