TABLE 2 

Transposition frequencies of DDE mutants

PlasmidIS26TargetTransposition frequencya
MeanNo.bRange
pRMH977IS26R3881.7 × 10−735.1 × 10−8–3.7 × 10−7
pRMH978IS26-FS-LcR388<1.8 × 10−83<1.1 × 10−8–<1.9 × 10−8
pRMH987IS26 D82LR388<5.3 × 10−83<4.3 × 10−8–<7.1 × 10−8
pRMH988IS26 D142LR388<3.4 × 10−93<2.8 × 10−9–<4.2 × 10−9
pRMH989IS26 E177LR388<4.4 × 10−83<1.8 × 10−8–<5.9 × 10−8
pRMH977IS26R388::IS262.1 × 10−431.9 × 10−4–2.4 × 10−4
pRMH978IS26-FS-LcR388::IS268.4 × 10−637.3 × 10−6–9.2 × 10−6
pRMH987IS26 D82LR388::IS266.4 × 10−634.8 × 10−6–9.6 × 10−6
pRMH988IS26 D142LR388::IS266.4 × 10−633.5 × 10−6–1.1 × 10−5
pRMH989IS26 E177LR388::IS269.7 × 10−634.3 × 10−6–2.0 × 10−5
  • a Transposition frequency expressed as cointegrates per transconjugant.

  • b Number of independent determinations.

  • c IS26-FS-L is an IS26 tnp26 frameshift mutant.