TABLE 3 

Parameters of intestinal permeability and low-grade inflammation in SIHUMI, SIHUMIw/oCra, or Cra mice after 4 weeks of high-fat diet feeding

ParameteraResult for miceb
SIHUMIw/oCraSIHUMICra
Intestinal permeability
    FITC-dextran concn (µg/ml)c4.55 ± 0.624.00 ± 1.203.29 ± 0.28
    Zo1 relative expression in:
        Jejunum1.00 ± 0.371.32 ± 0.421.71 ± 0.59d
        Ileum1.00 ± 0.080.87 ± 0.081.00 ± 0.08
        Colon1.00 ± 0.121.99 ± 0.09***1.91 ± 0.24**
    Ocln relative expression in:
        Jejunum1.00 ± 0.080.80 ± 0.120.94 ± 0.14
        Ileum1.00 ± 0.060.90 ± 0.070.77 ± 0.05*
        Colon1.00 ± 0.071.56 ± 0.13**1.10 ± 0.07
Low-grade inflammation
    LPS concn (IU/ml)c0.06 ± 0.030.02 ± 0.02ND
    Tnfa relative expression in:
        Jejunum1.00 ± 0.140.58 ± 0.170.71 ± 0.06
        Ileum1.00 ± 0.140.97 ± 0.120.43 ± 0.06**
        Colon1.00 ± 0.130.64 ± 0.09*1.21 ± 0.19
        Liver1.00 ± 0.081.99 ± 0.24**1.24 ± 0.15
        eWAT1.00 ± 0.131.41 ± 0.151.78 ± 0.28*
  • a FITC-dextran, 4 kDa fluorescein isothiocyanate-dextran; LPS, lipopolysaccharides; Ocln, occludin gene; Tnfa, tumor necrosis factor alpha gene; Zo1, zonula occludens-1 gene. Gene expression of Zo1, Ocln, and Tnfa was analyzed in the indicated tissues.

  • b Values are means ± SEM (n = 7 to 9). ND, not detected. *, P < 0.05, **, P < 0.01, and ***, P < 0.001, for obese SIHUMI or Cra mice versus less obese SIHUMIw/oCra mice (reference).

  • c Determined in peripheral blood plasma.

  • d n = 5.