TABLE 1 

Strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
Strains
    MG1655F λ rph-1This laboratory
    PK9416MG1655 ΔarcA4
    PK9483MG1655 PicdA(-58GGTGA-54)-lacZ4
    PK9484PK9416 PicdA(-58GGTGA-54)-lacZ4
    PK9494MG1655 PicdA(-58GGTGA-54, 19TG20)-lacZThis study
    PK9495PK9416 PicdA(-58GGTGA-54, 19TG20)-lacZThis study
    PK9486MG1655 PicdA(-58GGTGA-54, 8TG9)-lacZThis study
    PK9487PK9416 PicdA(-58GGTGA-54, 8TG9)-lacZThis study
    PK9496MG1655 PicdA(-58GGTGA-54, -4TG-3)-lacZThis study
    PK9497PK9416 PicdA(-58GGTGA-54, -4TG-3)-lacZThis study
    PK9915MG1655 PicdA(-58GGTGA-54, 29AACA32)-lacZThis study
    PK9916PK9416 PicdA(-58GGTGA-54, 29AACA32)-lacZThis study
    PK9917MG1655 PicdA(-58GGTGA-54, -15ACA-13)-lacZThis study
    PK9918PK9416 PicdA(-58GGTGA-54, -15ACA-13)-lacZThis study
    PK9924MG1655 PicdA(-58GGTGA-54, -5A)-lacZThis study
    PK9925PK9416 PicdA(-58GGTGA-54, -5A)-lacZThis study
    PK9941MG1655 PicdA(-58GGTGA-54, 7A)-lacZThis study
    PK9942PK9416 PicdA(-58GGTGA-54, 7A)-lacZThis study
    PK9943MG1655 PicdA(-58GGTGA-54, 7A, -5A)-lacZThis study
    PK9944PK9416 PicdA(-58GGTGA-54, 7A, -5A)-lacZThis study
    PK10967MG1655 PicdA(-58GGTGA-54, 7A, -4TG-3)-lacZThis study
    PK10968PK9416 PicdA(-58GGTGA-54, 7A, -4TG-3)-lacZThis study
    BW25993lacIq ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD7845
    PK9970PK9483 arcA::catThis study
    PK9973PK9915 arcA::catThis study
    PK9971PK9924 arcA::catThis study
    PK9972PK9943 arcA::catThis study
    PK9980PK9483 arcA-D54A::catThis study
    PK9983PK9915 arcA-D54A::catThis study
    PK9981PK9924 arcA-D54A::catThis study
    PK9982PK9943 arcA-D54A::catThis study
    PK9975PK9483 arcA-D54E::catThis study
    PK9978PK9915 arcA-D54E::catThis study
    PK9976PK9924 arcA-D54E::catThis study
    PK9977PK9943 arcA-D54E::catThis study
Plasmids
    pKD46Phage λ gam-bet-exo genes under ParaB controlB. L. Wanner
    pKD13FRT-kan-FRTK. A. Datsenko and B. L. Wanner
    pKD32FRT-cat-FRTB. L. Wanner
    pPK7035kan gene from pHP45Ω and BamHI-NdeI fragment from pRS1553 into pBR32237
    pPK9476pPK7035 PicdA(-58GGTGA-54)-lacZ4
    pPK9477pPK7035 PicdA(-58GGTGA-54 19TG20)-lacZThis study
    pPK9908pPK7035 PicdA(-58GGTGA-54 8TG9)-lacZThis study
    pPK9909pPK7035 PicdA(-58GGTGA-54 -4TG-3)-lacZThis study
    pPK9913pPK7035 PicdA(-58GGTGA-54 29AACA32)-lacZThis study
    pPK9914pPK7035 PicdA(-58GGTGA-54 -15ACA-13)-lacZThis study
    pPK15001pPK7035 PicdA(-58GGTGA-54, 7A, -4TG-3)-lacZThis study
    pPK9965arcA in pBR322This study
    pPK9966BamHI FRT-cat-FRT in pPK9965This study
    pPK9431Apr; His6-arcA cloned into the NheI and XhoI sites of pET-21d4