TABLE 3 

BLAST output of three unique regions from the mock communities against the databasea

BLAST query (accession no.:position), targetb% IdentitycAlignment lengthNo. of:QueryTargetAccession no.
MMdGapsStartEndStartEnd
Query—B. stercoris ATCC 43183  (NZ_DS499676.1:176961–207558)
    B. stercoris ATCC 43183100.0030,59800130,598176,961207,558NZ_DS499676.1
    B. vulgatus PC51099.9630,599102130,59830,5971NZ_ADKO01000036.1
    B. uniformis ATCC 849299.9530,60293130,598176,746207,345NZ_DS362245.1
    B. cellulosilyticus CL02T12C1999.8013,767233113,764624,314610,549NZ_JH724089.1
    B. vulgatus ATCC 848299.6625,4967610125,4912,046,6252,021,136NC_009614.1
    P. merdae ATCC 4318499.6125,5008312125,491117,63992,147NZ_DS264524.1
Query—B. fragilis HMW 616  (NZ_JH815527.1:1–13248)
    B. fragilis HMW 616100.0013,24800113,248113,248NZ_JH815527.1
100.0013,24800113,24880,75067,503NZ_JH815526.1
    P. merdae ATCC 4318499.9913,24810113,248356,073342,826NZ_DS264540.1
    Bacteroides sp. strain 4_3_47FAA99.9913,24810113,248561,549548,302NZ_JH114362.1
    B. coprocola DSM 1713689.098,440800874,87513,2488,64417,028NZ_DS981488.1
    B. plebeius DSM 1713589.098,440800874,87513,248241,574249,958NZ_DS990119.1
    B. finegoldii CL09T03C1086.885,349638467,93513,24882,42177,102NZ_JH951901.1
Query—B. faecis MAJ27  (NZ_AGDG01000049.1:1–12502)
    B. faecis MAJ27100.0012,50200112,502112,502NZ_AGDG01000049.1
    B. plebeius DSM 1713599.9812,50220112,50228,01915,518NZ_DS990120.2
    B. intestinalis DSM 1739399.9812,50221112,50214,7482,248NZ_ABJL02000003.1
    Bacteroides sp. strain D2299.8712,50204112,50235,21022,725NZ_GG774809.1
    P. merdae CL03T12C3298.718,73111216039,333139,124130,395NZ_JH976456.1
    Bacteroides sp. strain 9_1_42FAA98.679,24112012,51211,75225,55234,789NZ_EQ973174.1
  • a Boldface indicates strains from a natural ecosystem.

  • b All species belong to Bacteroides or Parabacteroides.

  • c % Identity was rounded to the closest hundredth of a percent.

  • d MM, mismatches.