TABLE 4 

Production of FAEEs and 3-HP in different strains holding different versions of ACC1

Strain(Over)expressed genes (HIS3 and URA3)aTotal FAEE production (mg/liter)Total 3-HP production (mg/liter)
CB2H1ws2 and —b4.7 ± 0.7
CB2H2ws2 and ACC1wt5.6 ± 0.8
CB2H3ws2 and ACC1ser1157ala9.5 ± 0.9
CB2H4ws2 and ACC1ser659ala,ser1157ala15.8 ± 2.5
HPY15mcrCa and —82.2 ± 2.2
HPY16mcrCa and ACC1wt126.8 ± 5.9
HPY17mcrCa and ACC1ser1157ala210.9 ± 9.9
HPY18mcrCa and ACC1ser659ala,ser1157ala279 ± 19.8
  • a Gene (over)expressed in HIS3 and URA3 based plasmid.

  • b No gene was inserted for (over)expression.