TABLE 3 

Oligonucleotides used in this study

PrimerOligonucleotide sequence (5′ → 3′)aSource
mexYUP-FATTAAGAGCTCTACCGCCAGGCTGThis study
mexYUP-RGCTGCATCTAGATCGTAGCGTTCTCThis study
mexYDN-FAGTCGATCTAGATGCCCCTAGCGAAACThis study
mexYDN-RGCAGACAAGCTTCTGGCCGACTATCThis study
mexYscreen-FTGTTCCGCAATCCGCATCThis study
mexYscreen-RGCGTAGCCGATCATCTGTCThis study
mexY-3138-FGGCTCGAAGCTTATGGCTCGTTTCTTCThis study
mexY-3138-RATCTAGGATCCTCAGGCTTGCTCCGTGThis study
mexY-58-FTTATACAAGCTTCGACGCCCCCTCACCGCTThis study
mexY-58-RTGGCGGTCATTTGGTTGACCThis study
mexYseq-F1GCCTGAAGATCGTCGAGTCThis study
mexYseq-F2CAAGCTGACCTCGATGAACCTThis study
mexYseq-F3CGATCAACGTGCTGACCATGTThis study
mexYseq-R4CAGTCCCTGCATCAATTGCTThis study
mexYseq-R5ATCTCGTCCATGCTCACGCThis study
mexY-K79A-FGGCCTGCTCTACACCGCGGCCACCAGCAGCACThis study
mexY-K79A-RGTGCTGCTGGTGGCCGCGGTGTAGAGCAGGCCThis study
mexY-D133A-FTGGAGAAGGCGGCGGCCAGCATCCAGCTGATThis study
mexY-D133A-RATCAGCTGGATGCTGGCCGCCGCCTTCTCCAThis study
mexY-D133S-FTGGAGAAGGCGGCGTCCAGCATCCAGCTGATThis study
mexY-D133S-RATCAGCTGGATGCTGGACGCCGCCTTCTCCAThis study
mexY-Y613A-FGGCGGCTTCAGCCTGGCCGGCGACGGCACCAGThis study
mexY-Y613A-RCTGGTGCCGTCGCCGGCCAGGCTGAAGCCGCCThis study
  • a Restriction endonuclease cleavage sites are underlined. Site-directed mutations are bolded and italicized.