TABLE 2 

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionaReference or source
E. coli strains
    DH5αφ80dlacZΔM15 endA1 recA1 hsdR17 (rK mK+) supE44 thi-1 gyrA96 relA1 F Δ(lacZYA-argF) U16949
    S17-1thi pro hsdR recA Tra+50
P. aeruginosa strains
    K767PAO1 prototroph wild type51
    K1525K767ΔmexXY52
    K3315K767ΔmexYThis study
Plasmids
    pEX18TcBroad-host-range gene replacement vector; sacB Tcr53
    pCL8pEX18Tc::ΔmexYThis study
    pCL9pEX18Tc::mexYThis study
    pRK415P. aeruginosa-E. coli shuttle cloning vector; Tcr54
    pCL10pRK415::mexYWTThis study
    pCL11pRK415::mexYS16FThis study
    pCL12pRK415::mexYR166CThis study
    pCL13pRK415::mexYR184HThis study
    pCL14pRK415::mexYG216DThis study
    pCL15pRK415::mexYV339MThis study
    pCL16pRK415::mexYP562SThis study
    pCL17pRK415::mexYA960TThis study
    pCL18pRK415::mexYK79AThis study
    pCL19pRK415::mexYD133AThis study
    pCL20pRK415::mexYD133SThis study
    pCL21pRK415::mexYY613AThis study
  • a Tcr, tetracycline resistance; WT, wild type.