TABLE 1

Calculation of butanol formed through the hot channel by using the reassimilation ratio of acetate to butyrate

StrainGrowth
ratec
(h−1)
Metabolite produced∫rACUPVOL/
∫rBUUPVOLe
Molar equivalent
of butanol formed
through hot and
cold channels (mmol)
% of butanol
formed through
hot and cold
channels
Ratio of
hot- to
cold-channel
butanol
(HotBuOH/
ColdBuOH;
mol/mol)
MetaboliteConc
(g/liter)
Molar
equivalent
(mmol)
Yieldd
(mol/mol)
ColdBuOHfHotBuOHgColdBuOHHotBuOH
ATCC 8240.33Acetone5.493.0
Butanol11.8159.20.435.553.6105.634662.0
EKW0.28Acetone7.7132.6
Butanol17.2232.10.523.381.7150.435651.8
BKW0.38Acetone8.0137.7
Butanol15.2205.10.486.578.1127.038621.6
BEKW0.36Acetone2.441.3
Butanol16.0215.90.541.429.3186.614866.4
PEKW0.19Acetone2.237.9
Butanol5.979.60.242.125.054.631692.2
BEKW*a0.29Acetone2.136.2
Butanol18.4248.20.671.126.6221.711898.3
BEKW**b0.27Acetone1.525.8
Butanol18.9255.00.711.81 × 10512.9242.159518.8
  • a  BEKW*, BEKW(pPptbAAD).

  • b  BEKW**, BEKW(pPthlAAD**).

  • c  Growth rate was calculated only for those cells in log phase.

  • d  Butanol yield on glucose.

  • e  Reassimilation ratio of acetate to butyrate (∫rACUPVOL/∫rBUUPVOL). Integration of the flux was carried out using a function of the calculus/integrate in the Origin 7.0 software program (OriginLab Corp., Northampton, MA). See Table S1 in the supplemental material for details on the calculation of the ∫rACUPVOL/∫rBUUPVOL value.

  • f  Butanol formed through the cold channel: ColdBuOH (mmol) = [molar equivalent of butanol produced (mmol) × ∫rACUPVOL/(∫rACUPVOL + ∫rBUUPVOL)]/2 + [molar equivalent of butanol produced (mmol) × ∫rBUUPVOL/(∫rACUPVOL + ∫rBUUPVOL)].

  • g  Butanol formed through the hot channel: HotBuOH (mmol) = molar equivalent of butanol (mmol) − ColdBuOH (mmol).