TABLE 4

Expression ratios of genes encoding Msr (methanol-specific regulatory) family and putative Mre family regulatory proteins in the acetate-grown ΔmreA strain versus the WWM75 strain of M. acetivorans

GeneTranscript abundance (ΔmreA strain/WWM75)a
MA0459 (msrA)3.06
MA0460 (msrB)1.93
MA0862 (msrF)8.66
MA1671 (mreB)8.64 (10.2 ± 2.6)b
MA3129 (mreC)8.67 (13.0 ± 3.8)b
MA3130 (mreD)363.95 (1440 ± 430)b
MA4167 (msrH)11.77 (19.0 ± 5.2)b
MA4383 (msrC)27.93
  • a Determined by RNA-Seq (see Table S2 in the supplemental material) unless noted otherwise, as indicated in footnote b.

  • b Determined by qRT-PCR, calculated using the ΔΔCT method with the 16s rRNA gene used as an invariant control. Values represent the averages and standard deviations of the results of two biological replicate experiments assayed in triplicate. Primer and probe sequences are listed in Table S4 in the supplemental material.