TABLE 3

Expression ratios of genes in methylotrophic pathways for the acetate-grown ΔmreA strain versus the WWM75 strain of M. acetivorans

GeneTranscript abundance (ΔmreA strain/WWM75)a
Methyl transfer branch
 MA0144–MA0145 (mtmBC-1)3.95–3.13
 MA0146 (mtbA)4.24
 MA0455–MA0456 (mtaBC-1)0.88–0.49
 MA0527, MA0532 (mtbBC-1)0.68–1.70
 MA0528–MA0529 (mttBC-1)0.53–0.57
 MA0859 (mtsD)1.36
 MA0931–MA0932 (mttBC-2)6.12–9.27
 MA0933–MA0934 (mtbBC-2)8.2–10.60
 MA1615 (mtaA2)0.92
 MA1616–MA1617 (mtaBC-3)1.21–1.22
 MA2424–MA2425 (mtbBC-3)0.78–0.95
 MA2971–MA2972 (mtmBC-2)1.37–1.44
 MA4379 (mtaA-1)7.83
 MA4384 (mtsF)1.00
 MA4558 (mtsH)0.93
Oxidative branch
 MA0010 (ftr)4.98 (7.07 ± 1.52)b
 MA0304–MA0309 (fmdEFADCB)7.81–10.09
 MA0304 (fmdE)14.8 ± 4.0b
 MA0309 (fmdB)11.3 ± 2.4b
 MA1710 (mch)1.85
 MA3733 (mer)6.55 (8.70 ± 2.11)b
 MA4430 (mtd)1.97
Energy conversion
 MA1494–MA1507 (fpoPABCDHIJJKLMNO)6.35–13.64
 MA1495 (fpoA)9.34 ± 0.92b
 MA1498 (fpoD)11.9 ± 1.5b
 MA3732 (fpoF)3.91
  • a Determined by RNA-Seq unless noted otherwise, as indicated in footnote b.

  • b Determined by qRT-PCR, calculated using the ΔΔCT method with the 16s rRNA gene used as an invariant control. Values represent the averages and standard deviations of the results of two biological replicate experiments assayed in triplicate. Primer and probe sequences are listed in Table S4 in the supplemental material.