TABLE 1

Expression of mreA during growth of wild-type M. acetivorans strain WWM75 on acetate, methanol, or trimethylamine

SubstrateExpression level
qRT-PCR (ΔΔCT)aGus (nmol·min−1 ⋅ mg protein−1)b
Acetate38.0 ± 12.19.70 ± 2.26
TMA2.22 ± 0.680.325 ± 0.075
Methanol1.00 ± 0.170.276 ± 0.024
  • a Calculated using the threshold cycle (ΔΔCT) method with the 16s rRNA gene used as an invariant control. The ΔCT values for methanol cells were used as the calibrator. Values represent the averages and standard deviations of the results of two biological replicate experiments assayed in triplicate. Primer and probe sequences are listed in Table S4 in the supplemental material.

  • b β-Glucuronidase activity was measured in cell lysate collected at mid-log growth. Values represent the averages and standard deviations of the results of three biological replicate experiments.