TABLE 2 

BLAST and Pfam analyses of putative phosphoregulatory system present in E. faecium, E. casseliflavus, and E. gallinarum

Representative
locusa
Pfam hit
(E value)b
BLASTP best hitc
S. pneumoniae
TIGR4
B. subtilis
168
EFPG_02020NoneNoneNone
EFPG_02021LytR_cpsA_psr (2.7e-49)SP_1942, transcriptional
regulator (9e-75, 65%, 95%)d
Membrane-bound transcriptional
regulator LytR (1e-83, 67%, 91%)
EFPG_02022Wzz (1.1e-19)Capsular polysaccharide
biosynthesis protein
Cps4C (6e-15, 48%, 81%)
YwqC, modulator of YwqD
protein tyrosine
kinase activity (3e-45, 60%, 84%)
EFPG_02023CbiA (1.8e-14)Capsular polysaccharide
biosynthesis protein
Cps4D (9e-42, 56%, 87%)
PtkA/YwqD, protein
tyrosine kinase
(7e-74, 71%, 93%)
EFPG_02024NoneCapsular polysaccharide
biosynthesis protein
Cps4B (2e-25, 49%, 88%)
YwqE, protein tyrosine
phosphatase
(5e-68; 61%, 100%)
  • a  From E. faecium 933.

  • b  Pfam hits with E values of ≤10−5 are shown.

  • c  Values in parentheses are E value, % similarity, and % query coverage.

  • d  The second-best hit was capsular polysaccharide biosynthesis protein Cps4A (7e-31, 79%, 55%).