TABLE 1

Genes differentially regulated during alkalinizationa

Annotation and geneExpression (fold)Function of encoded protein
Orf19ach1WT-Glu
Arginine metabolism
CAN21118.966.6Basic amino acid permease
CAR13,9348.13.3Arginase (Arg → urea-ornithine)
CAR25,64110.33.1Ornithine transaminase (Orn → Glu)
CAN1974.23.0Basic amino acid permease
ARG17,4690.180.31Argininosuccinate synthetase
ARG46,6890.630.31Argininosuccinate lyase
ARG35,6100.110.14Ornithine carbamoyltransferase
DUR1,27801.30.12Urea amidolyase
DUR37810.970.03Putative urea transporter
Methionine/cysteine/sulfate metabolism
MET22,6180.150.29Homoserine acetyltransferase
MET104,0760.590.28Sulfite reductase
MET45,3120.280.28Transcription factor for MET/CYS
MET155,6451.10.19Acetylhomoserine aminocarboxypropyl transferase
MET149460.330.18Adenylyl-sulfate kinase
MET15,8420.260.12Uroporphryin methyltransferase
MET35,0250.250.10ATP sulfurylase
Transporters/channels
JEN17,44715.799.9Lactate transporter
HGT137,0933.412.2Glucose transporter
HIP13,1952.110.3Similar to amino acid permeases
CDR31,31317.79.4ABC transporter of phospholipids
HGT23,6683.35.6Glucose transporter
DIP52,9420.944.0Dicarboxylic acid permease
GIT21,9785.64.0Putative glycerophosphoinositol permease
HGT166,1412.73.2Glucose transporter
AQY12,84922.73.1Aquaporin water channel
341.90.31Putative glycerophosphoinositol permease
3,1200.380.27Putative PDR family transporter
HAK16,2490.350.19Putative potassium transporter
MEP11,6141.80.12Ammonium permease
MEP25,6720.490.05Ammonium permease
Alternative carbon utilization
ACS11,74349.719.1Acetyl-CoA synthetase
FDH16380.8211.7Formate dehydrogenase
PXP21,65515.19.5Acyl-CoA oxidase (β-oxidation)
FAA27,3794.66.8Fatty acyl-CoA ligase (β-oxidation)
ICL16,8440.885.3Isocitrate lyase (glyoxylate cycle)
MLS14,8330.904.4Malate synthase (glyoxylate cycle)
FOX21,2880.693.33-Hydroxyacyl-CoA epimerase (β-oxidation)
  • a  Ratios given are the expression levels in alkalinizing cultures relative to the levels for the nonalkalinizing ach1∆ mutant (ach1∆ column) or the nonalkalinzing high-glucose condition (WT-Glu column). Shown are >3-fold changes up (bold) or down (italic).