Strains and plasmids used

Strain or plasmidGenotype or phenotypeReference
R. palustris strains
 CGA009Wild-type strain; spontaneous Cmr derivative of CGA00117
 CGA676NifA*; produces H2 in the presence of NH4+7
 CGA669ΔcbbLS::Kmr ΔcbbM mutant of CGA0097
 CGA679ΔcbbLS::Kmr ΔcbbM mutant of CGA6767
 pBBPgdhGmr; pBBR1MCS-5 with RPA0944 promoter between KpnI and XhoI sites7
 pBBPcbbLSXGmr; cbbLSX with native ribosomal binding site cloned into pBBPgdh7