TABLE 2

Genes identified by Tn-seq exhibiting strong mutant hypersensitivity to tobramycina

LocusGeneFunctionNo. of normalized reads (no. of hits with reads)bSelection ratiobPreviously identifiedcNo. of mutants testeddMICe
PregrowthGrowth without TobGrowth with Tob
None (PAO1)1.0
PA3303 (control)1,888 (17)2,062 (20)1,703 (20)0.8311.0
PA0392Conserved hypothetical644 (5)1,374 (5)0 (0)0No2f0.25
PA4077Transcriptional regulator1,572 (3)1,230 (3)0 (0)0No1f0.25
PA5199 amgS Two-component sensor764 (18)663 (20)0 (0)0Yes0.125
PA5366 pstB Phosphate transport1,748 (8)761 (8)0 (0)0Yes0.25
PA5200 amgR Two-component response regulator752 (9)396 (12)2 (1)0.00Yes0.063
PA0016 trkA Potassium uptake1,089 (16)823 (16)5 (2)0.01Yes0.25
PA4942 hflK Protease subunit2,436 (14)2,104 (16)30 (2)0.01Yes0.25
PA3014 faoA Fatty acid oxidation1,465 (23)1,227 (27)56 (5)0.05No50.25
PA4398Two-component sensor862 (19)633 (19)30 (2)0.05Yes0.25
PA5528Hypothetical2,363 (12)2,839 (13)161 (4)0.06Yes0.125
PA1805 ppiD Peptidyl-prolyl isomerase3,920 (29)4,583 (29)397 (12)0.09No50.25
PA3016Hypothetical1,630 (9)840 (8)87 (1)0.10Yes0.125
PA4223Transport638 (19)790 (19)106 (10)0.13No30.25
PA4960Amino acid metabolism916 (15)507 (17)80 (2)0.16No20.25
PA0374 ftsE Cell division4,583 (8)4,366 (8)705 (4)0.16No30.125g
PA3013 foaB Fatty acid oxidation3,632 (29)2,925 (32)485 (14)0.17Yes0.25
PA5471Hypothetical655 (8)755 (8)129 (4)0.17Yes0.25
PA0502Biotin biosynthesis1,131 (9)627 (9)108 (3)0.17No10.25
PA3194 edd Carbohydrate metabolism253 (10)161 (11)29 (5)0.18No10.125
PA1775 cmpX Cytoplasmic membrane protein1,670 (13)747 (11)134 (7)0.18No30.25
PA0427 oprM Multidrug efflux1,877 (15)1,427 (15)298 (6)0.21Yes0.25
PA4222Transport551 (16)333 (18)74 (6)0.22No20.25
PA5369 pstS Phosphate transport1,037 (10)631 (9)164 (3)0.26No30.25g
PA2018 mexY Multidrug efflux3,025 (45)2,761 (46)811 (40)0.29Yes0.25
PA5285Hypothetical1,293 (4)1,559 (4)466 (4)0.30No40.25
PA2604Conserved hypothetical2,736 (8)2,162 (9)649 (8)0.30No30.25
PA2019 mexX Multidrug efflux2,403 (20)2,488 (21)930 (18)0.37Yes0.25
PA4050 pgpA Phospholipid biosynthesis1,931 (6)1,214 (7)483 (4)0.40No30.125
  • a  Genes whose mutation caused a 4-fold or greater MIC change are listed.

  • b  See text for method of calculation. Tob, tobramycin.

  • c  Reference 7.

  • d  Number of independent mutants tested. Thirty-four of the 42 mutants examined were confirmed by resequencing, including at least one representative for each gene.

  • e  MIC values (in micrograms per milliliter) for representative mutants as measured in this study or in reference 7.

  • f  Includes one deletion mutant.

  • g  Phenotype possibly due to expression of hybrid proteins.