TABLE 1 

Summary of total RNA-Seq reads mapped

Infection and sample no.No. (%) of reads mappeda
Human genomeToxoplasma genome
Mock
    128,303,437 (99.5)136,537 (0.5)
    226,656,452 (99.4)153,263 (0.6)
    313,637,605 (99.2)105,372 (0.8)
    413,007,385 (99.3)98,286 (0.7)
    55,280,417 (99.2)40,551 (0.8)
    615,675,192 (99.5)85,248 (0.5)
    710,225,078 (99.3)68,095 (0.7)
 
RH
    117,541,338 (76.3)5,436,742 (23.7)
    215,710,664 (72)6,111,477 (28)
    313,401,169 (71.5)5,352,720 (28.5)
    413,606,269 (75.2)4,497,829 (24.8)
    51,895,604 (67.1)929,937 (32.9)
    61,501,296 (77.5)436,416 (22.5)
    71,118,902 (63.9)631,314 (36.1)
 
RHΔmyr1
    118,334,239 (78.3)5,080,247 (21.7)
    217,603,181 (72.7)6,625,567 (27.3)
    312,889,729 (64)7,253,952 (36)
    415,546,151 (71.9)6,078,441 (28.1)
    53,996,692 (63.7)2,276,057 (36.3)
    61,246,788 (68.1)583,369 (31.9)
    72,284,809 (61.8)1,410,089 (38.2)
 
RHΔmyr1::MYR1
    118,645,106 (79.3)4,864,519 (20.7)
    217,179,730 (72.2)6,629,830 (27.8)
    313,579,524 (67.5)6,531,328 (32.5)
    48,443,874 (69.5)3,713,665 (30.5)
    55,650,821 (79.2)1,488,299 (20.8)
    63,624,860 (52.3)3,299,922 (47.7)
    71,409,132 (49.3)1,451,964 (50.7)
 
RHΔasp5
    110,592,764 (87.4)1,523,876 (12.6)
    210,290,292 (75.1)3,411,275 (24.9)
    35,057,673 (76.8)1,523,876 (23.12)
    47,498,195 (77.9)2,121,971 (22.1)
    53,978,935 (75.5)1,291,803 (24.5)
 
RHΔasp5::ASP5
    110,177,793 (65.2)5,433,843 (34.8)
    29,791,658 (81.4)2,235,700 (18.6)
    33,690,117 (63.5)2,123,616 (36.5)
    46,088,041 (86.9)921,158 (13.1)
    55,679,354 (82.4)1,216,067 (17.6)
  • a Shown are the numbers of raw reads mapped to human and Toxoplasma exons using CLC Genomics and the percentage of all reads mapping to these genomes.