TABLE 1 

Summary of best-fit diffusion coefficients and fractional populations

EF-Tu or ribosome typeaDmean (µm2/s)bfslowcDslow (µm2/s)Dfast (µm2/s)
Normal growth conditions
    EF-Tu WTd2.02 ± 0.190.60 ± 0.051.0 ± 0.24.9 ± 1.2
    Ribosome WTe0.4 ± 0.10.7 ± 0.050.1 ± 0.11.2 ± 0.5
    EF-TuL148A mutantd3.1 ± 0.30.3 ± 0.051.2 ± 0.54.5 ± 1.0
After Rif treatment
    EF-Tu WTd3.5 ± 0.40.35 ± 0.051.5 ± 0.54.9 ± 1.5
    EF-TuL148A mutantd5.2 ± 0.40.1 ± 0.051.9 ± 1.25.6 ± 1.2
  • a Normal growth conditions were used, except for measurements after rifampin (Rif) treatment as noted.

  • b Mean diffusion coefficient estimated from first two points of MSD plot (Fig. 2).

  • c Best-fit fractional population of the more slowly diffusing state. The fractional population of the more rapidly diffusing state is ffast = 1 – fslow.

  • d C terminus labeled with mEos2.

  • e 30S subunits labeled by expression of the ribosomal protein S2-mEos2.