TABLE 1 

Sites of mutations in spontaneous Gac mutants sampled from cultures of wild-type P. protegens Pf-5 and derivativesa

StrainReplicateColonygacSbgacAb
M1AT254ST307PA447VA463DL476FQ727*2419^2420 insG*Δ500-504Δ2422-2426RBSV4EV4GI29NT103IQ155RK190R
Pf-511X
12X
21X
22X
31X
32X
ΔofaA11X
12X
21c
22X
31X
32X
ΔpltA11X
12X
21X
22X
31X
32X
LK14711X
12X
21X
22X
31X
32X
  • a Pprotegens Pf-5 and derivatives (ΔofaA, ΔpltA, and the 6-fold mutant LK147) were each grown in three replicate flasks containing NYB for 4 days, dilutions of the cultures were spread on LMA, and two exoprotease-deficient colonies were selected from each replicate. gacS and gacA were PCR amplified and sequenced to map mutations.

  • b Abbreviations of amino acids show the results of point mutations in the gacS or gacA gene. RBS indicates a mutation in the predicted ribosome binding site. An asterisk indicates a stop codon introduced by a point mutation or by an insertion.

  • c No mutation in gacS or gacA was found in this isolate.