TABLE 1 

Recombinant proteins of C. neoformans strain H99 expressed in E. coli and used for testing as vaccines

CNAG no.NameDescriptionRegion
expressed
VectorExpressed
protein mass
(kDa)
% of identity to
C. gattii R265
homologa
Human
homologyb
No. of
RNA-seq
readsc
CNAG_05799 Cda1Chitin deacetylase20–374pET20042.390None139,907
CNAG_01230 Cda2Chitin deacetylase20–378pET20043.888None5,835
CNAG_01239 Cda3Chitin deacetylase19–336pET19b37.9875.3864
CNAG_06291 Fpd1Polysaccharide deacetylase20–249pET20029.392None1,511
CNAG_01019 Sod1Superoxide dismutase2–154pET20020.1705−6270,771
CNAG_00776 MP88Mannoprotein23–294pET20032.994None24,244
  • a C. gattii strain R265 protein sequences were retrieved from the Ensembl Fungi website (http://fungi.ensembl.org/index.html ). Regions of C. gattii protein comparable to C. neoformans protein sequence expressed in E. coli were aligned to calculate the percentage of identity.

  • b Homology to the human protein with the highest similarity (BLASTp analysis); the smaller the numerical value, the greater the similarity.

  • c Shown are the average numbers of normalized RNA-seq reads recorded for two strains of Cryptococcus isolated directly from infected human patients (GEO GenBank accession no. GSE51573 ) (28).