TABLE 1 

Genes identified by TraDIS that affect capsule production in PA45B

Gene nameLocus tagMutated geneCapsule synthesis disruptedbGenome positonProductLog FCc
mprAPA45B_3600++2953533–2954063DNA-binding transcriptional repressor7.28
neuAaPA45B_3235NA3339754–3340863N-Acylneuraminate cytidylyltransferase7.21
kpsCaPA45B_3240NA3336039–3337244Capsule polysaccharide export protein7.19
kpsEaPA45B_3243NA3330382–3331530Capsule polysaccharide export protein7.18
kpsDaPA45B_3242++3331554–333230Capsule polysaccharide export protein7.18
kpsMaPA45B_3231NA3344548–3345324Capsule polysaccharide transport protein7.16
kpsTaPA45B_3232NA3346382–3346918Capsule polysaccharide transport ATP-binding protein7.12
kpsSaPA45B_3239NA3336039–3337244Capsule polysaccharide export protein7.10
kpsFaPA45B_3244NA3329327–3330310Capsule polysaccharide export protein7.08
neuBaPA45B_3234NA3342182–3343222N,N′-diacetyllegion-aminic acid synthase7.00
neuDaPA45B_3233NA3343219–3343842Galactoside O-acetyl-transferase monomer7.00
typAaPA45B_2253++4361535–4363358GTP-binding protein6.46
kpsUaPA45B_3241NA3333240–3333980Capsule polysaccharide export protein5.38
rfaHaPA45B_2283++4327933–4328421DNA-binding transcriptional anti-terminator5.08
  • a Gene known to be involved in capsule expression.

  • b NA, not applicable, because the gene was not mutated.

  • c The log2 fold change in read counts from the test pool versus the control pool.