Exflagellation assay results and mosquito infections with WT and PfCDPK2 KO parasitesa

Parasite% stage V
Sex ratio
No. of
No. of
% infected mosquitoes
(no. infected/total
no. dissected)
Median no. of
1WT1.35ND108782.5 (33/40)4 (0–12)
PfCDPK2 KO C11.52ND1060 (0/42)0 (0)
PfCDPK2 KO C130.84ND1050 (0/40)0 (0)
2WT1.301:1.9 (70/130)102482.1 (23/28)5 (0–24)
PfCDPK2 KO C11.601:1.8 (87/160)4510 (0/37)0 (0)
3WT0.151:3 (21/63)101781.8 (27/33)3 (0–21)
PfCDPK2 KO C10.961:2 (100/211)1000 (0/31)0 (0)
4WT1.111:2.6 (56/144)1611678.9 (15/19)3 (0–10)
PfCDPK2 KO C10.531:2.1 (36/77)2600 (0/20)0 (0)
5WT1.351:2.2 (60/129)2029575 (9/12)2.5 (0–22)
PfCDPK2 KO C10.781:1.7 (85/147)2000 (0/15)0 (0)
  • a Data from the in vitro exflagellation assay, a semiquantitative measurement, and mosquito infections for WT and PfCDPK2 KO C1 parasite from five different biological experiments are tabulated here. The exflagellation centers were counted under a bright-field microscope with a 40× objective lens. The 40× fields used for counting the exflagellation centers had what appeared to be a similar number of total RBCs in the WT and PfCDPK2 KO parasites. Importantly, the same cultures of the parasites were used for feeding female Anopheles stephensi Nijmegen mosquitoes, and no infection was detected with the PfCDPK2 KO parasites in the five experiments. ND, not determined.