TABLE 2 

Affinity measurement by biolayer interferometrya

Gene/proteinKD (M)Ka (1/ms)Ka errorKd (1/s)Kd error
ydaM 3.54E−081.14E+047.61E+014.03E−049.48E−06
yhjH 3.53E−081.22E+048.79E+014.29E−041.18E−05
yhjK 3.80E−081.06E+041.67E+024.01E−042.33E−05
ydeH 8.11E−087.74E+032.05E+026.27E−043.38E−05
flhD NDNDNDNDND
yhjK/BSANDNDNDNDND
  • a Equilibrium dissociation constants (KD) were determined by biolayer interferometry using a BLItz system (ForteBio Inc.) according to the results of an advanced kinetics experiment. Ka, association rate constant; Kd, dissociation rate constant; KD, equilibrium dissociation constant of the reaction; ND, not determined.