TABLE 1 

The effect of MGE1 overexpression on the MICflu of several strainsd

StrainMICflu (μg/ml)
EtestBroth microdilution assay
MIC50MIC90
S. cerevisiae BY4742 EV6–88–1616
S. cerevisiae BY4742 MGE124–3216–3232–64
S. cerevisiae ira2Δ EV4–68–168–16
S. cerevisiae ira2Δ MGE1 12–1616–3216–32
S. cerevisiae yme1Δ EV12–168–1616–32
S. cerevisiae yme1Δ MGE1 32–4816–3232–64
S. cerevisiae opi1Δ EV12–168–168–16
S. cerevisiae opi1Δ MGE1 48–6432–6432–64
S. cerevisiae rho0 EV24–3216–3216–32
S. cerevisiae rho0MGE1>25632–6432–64
S. cerevisiae pdr5Δ EV0.250.5–10.5–1
S. cerevisiae pdr5Δ MGE1 0.750.5–11–2
S. cerevisiae upc2Δ EV4–64–84–8
S. cerevisiae upc2Δ MGE1 24–328–1616–32
S. cerevisiae tom70Δ EV6–88–168–16
S. cerevisiae tom70Δ MGE1 16–2416–3232–64
S. cerevisiae ecm10Δ EV6–88–1616–32
S. cerevisiae ecm10Δ MGE1 24–3232–6432–64
S. cerevisiae ssq1Δ EV2–4a2–4a2–4a
S. cerevisiae ssq1Δ MGE1 1–1.5abb
S. cerevisiae aft1Δ EV4–68–1616
S. cerevisiae aft1Δ MGE1 4–68–168–16
S. cerevisiae aft2Δ EV4–68–168–16
S. cerevisiae aft2Δ MGE1 32–4816–3232–64
S. cerevisiae BY47426–88–1616–32
S. cerevisiae fra1Δ 6–88–1616–32
C. glabrata HTL EV8 (16–24)c2–44–8
C. glabrata HTL pTDH3-CgMGE124 (48–64)c4–88–16
C. glabrata HTL pPGK1-CgMGE116 (48–64)c2–48–16
  • a Data were determined after 72 h on SCglu (latter only for Etest).

  • b —, data could not be determined due to low growth.

  • c RPMI medium with 0.2% (or 2%) glucose was used.

  • d Values were determined by Etest and broth microdilution analysis. MGE1, MGE1 overexpression; EV, empty vector control.