Fungal Isocyanide Synthases and Xanthocillin Biosynthesis in Aspergillus fumigatus

ABSTRACT Microbial secondary metabolites, including isocyanide moieties, have been extensively mined for their repertoire of bioactive properties. Although the first naturally occurring isocyanide (xanthocillin) was isolated from the fungus Penicillium notatum over half a century ago, the biosynthetic origins of fungal isocyanides remain unknown. Here we report the identification of a family of isocyanide synthases (ICSs) from the opportunistic human pathogen Aspergillus fumigatus. Comparative metabolomics of overexpression or knockout mutants of ICS candidate genes led to the discovery of a fungal biosynthetic gene cluster (BGC) that produces xanthocillin (xan). Detailed analysis of xanthocillin biosynthesis in A. fumigatus revealed several previously undescribed compounds produced by the xan BGC, including two novel members of the melanocin family of compounds. We found both the xan BGC and a second ICS-containing cluster, named the copper-responsive metabolite (crm) BGC, to be transcriptionally responsive to external copper levels and further demonstrated that production of metabolites from the xan BGC is increased during copper starvation. The crm BGC includes a novel type of fungus-specific ICS-nonribosomal peptide synthase (NRPS) hybrid enzyme, CrmA. This family of ICS-NRPS hybrid enzymes is highly enriched in fungal pathogens of humans, insects, and plants. Phylogenetic assessment of all ICSs spanning the tree of life shows not only high prevalence throughout the fungal kingdom but also distribution in species not previously known to harbor BGCs, indicating an untapped resource of fungal secondary metabolism.

M icrobial natural products or specialized metabolites have been extensively mined for their pharmaceutical potential since the serendipitous discovery of penicillin by Alexander Fleming in 1928 that brought about the Golden Age of antibiotics. Unfortunately, years of mining our soils for new antibiotics have seen a rapid exhaustion of this resource pool, leading to unacceptably high rediscovery rates of the same class of compounds. Coupled with the concerning rise of antibiotic resistance and dwindling options for new effective antibiotics, the field of drug discovery is being ushered into finding alternative ways to mine new and unique microbial compounds. Today, our deeper understanding of the pathways and specialized enzymes involved in microbial secondary metabolite biosynthesis has enabled us not only to mine the wealth of sequenced microbial genomes for novel and unique bioactive natural products but also to generate methods for a sustainable source of these compounds.
Isocyanides (or isonitriles) are a class of microbial secondary metabolites long pursued for their broad repertoire of pharmacological properties. Since the isolation of the first naturally occurring isocyanide, xanthocillin (compound 1), from Penicillium notatum in 1950 (1), diverse isocyanide-containing natural products have been isolated from marine and terrestrial sources, including marine sponges (2,3), nudibranch molluscs (4), bacteria (5)(6)(7)(8), and fungi (9)(10)(11) (Fig. 1A). Many marine isocyanides are nitrogenous terpenes known as isocyanoterpenes, where the terpenoid carbon scaffolds are adorned with various isocyanide, formamide, isocyanate, or isothiocyanate functional groups (3). Isocyanides from terrestrial sources, on the other hand, are mostly derived through modification of ␣-amino acids or indole alkaloids, which in some cases are glycosylated (5). The presence of an isonitrile moiety imparts unique biological (e.g., cytotoxic, antibacterial, and antiprotozoal) and chemical (e.g., transition metal coordination) properties and has enabled synthetic and biochemical (e.g., "click" chemistry) applications (12,13). In addition to industrial and pharmaceutical applications, naturally occurring bacterial isocyanides such as the diisonitrile SF2768 from Streptomyces thioluteus (7) have been shown to function as chalkophores: natural products involved in copper chelation and extracellular copper uptake. Due to their ability to form coordination complexes with various transition metals, there is growing evidence that isocyanides play an important role in pathogenesis of insect, plant, and human diseases (5,8,10).
Despite their broad range of applications and emerging impact on various hostpathogen interactions, relatively little is known about the biosynthesis or molecular switches governing the production of isocyanides, in contrast to the well-studied nonribosomal peptide and polyketide biosynthetic pathways. The first insights into microbial isocyanide biosynthesis were enabled by the discovery of bacterial synthases termed isonitrile synthases (ISNs) or isocyanide synthases (ICSs) (e.g., PvcA [6,14], IsnA [5,15], AmbI1/AmbI2 [16], and WelI1/WelI2 [16]) shown to be responsible for the conversion of amino groups into isonitrile moieties in aromatic amino acids (15,17). More recently, two new biosynthetic routes to bacterial isocyanide production have been reported: one that requires the activity of two modifying enzymes, a thioesterase homologue (ScoD) and a nonheme iron(II)-dependent oxidase (ScoE), identified in Mycobacterium tuberculosis to produce isonitrile lipopeptides (8), in addition to another that requires the activity of a typical nonribosomal peptide synthetase (SfaD), identified in S. thioluteus to produce the diisonitrile SF2768 (7).
Although fungi are known producers of various isocyanide compounds, including xanthocillin, the first isocyanide to be isolated, the biosynthetic origins of fungal isocyanides remain unknown. Production of xanthocillin-like isocyanides such as BU-4704 (18) and xanthoascin, an isocyanide first isolated from Aspergillus candidus (9,19), have been reported from Aspergillus fumigatus, a filamentous mold that is both a ubiquitous compost-loving carbon-nitrogen recycler and a highly fatal opportunistic pathogen of immunocompromised individuals (20). Given the emerging role of isocyanide compounds during pathogenesis of insect (5), plant (5,10), and human (8) pathogens, we sought to identify the biosynthetic origins of fungal isocyanides in this opportunistic pathogen.
Here we present the first fungal isocyanide biosynthetic pathway responsible for production of xanthocillin-like isocyanides and two previously undescribed melanocinlike dipeptides in A. fumigatus. Via genome mining, we identified four isocyanide synthases (ICSs) parsed across three biosynthetic gene clusters (BGCs) (Fig. 1B)   x a n A x a n B x a n C x a n D x a n E x a n F x a n G current in silico BGC prediction algorithms. Given the cryptic nature of these BGCs in A. fumigatus, we expanded our search for ICSs and found widespread occurrence of ICS homologues within the fungal kingdom, including distribution within fungal taxa not generally recognized to harbor secondary metabolite-producing BGCs. An exhaustive search for ICS-containing genes spanning the tree of life identified three ICS enzyme variations: homologues of monodomain isocyanide synthases (ICSs), putative twodomain isocyanide synthase-dioxygenases (ICS-DOX), and a novel group of multidomain putative isocyanide synthase-nonribosomal peptide synthases (ICS-NRPSs), which are found predominantly in fungal pathogens. We further demonstrate the production of isocyanides and transcription of their biosynthetic enzymes in A. fumigatus to be copper responsive, and we show that ICS overexpression induces cellular copper starvation.
Expansion of ICS domain proteins across the tree of life. Phylogenetic analysis of the ICS domain (PF05141)-containing proteins (from here on referred to as ICS proteins) showed that all four A. fumigatus Af293 ICS proteins group within the fungal clades ( Fig. 2A; see Fig. S3 in the supplemental material). The taxonomic diversity and tree topology of PF05141 suggest this domain has a complex evolutionary history, including multiple gene duplication, loss, and horizontal transfer events; however, the exact timing and locations of these events are unclear. In bacteria, ICS proteins are distributed mainly across Proteobacteria, Cyanobacteria, and Actinobacteria ( Fig. 2A and Fig. S3). In eukaryotes, ICS proteins are widespread across the fungal kingdom but are found only sporadically in 10 species of oomycetes, one species of Amoebozoa, and one species of moss ( Fig. 2A and Fig. S3).
Within the kingdom Fungi, ICS proteins are highly prevalent in the phylum Ascomycota; in addition, they are also found in four species of the phylum Basidiomycota ( Fig. 2A; see Fig. S3 and Table S1 in the supplemental material). In the Ascomycota, we find ICS proteins to be most common throughout the Pezizomycotina ( Fig. 2A and Fig. S3); however, they also occur in 20 species of Saccharomycotina, including several clinically important Candida species and in the economically important baker's yeast, Saccharomyces cerevisiae ( Fig. 2A and Table S1). In these latter fungi, the ICS-homologous protein is known as Dit1, which has been shown to participate in the biosynthesis of the dipeptide dityrosine, an ascospore wall component (see below) (27).
Our analysis revealed that ICS proteins commonly exhibit two distinct protein domain architectures in bacteria but three in eukaryotes: (i) single-domain ICSs found Isocyanide Synthases in Aspergillus fumigatus ® in both bacteria (e.g., P. aeruginosa PvcA) and eukaryotes (e.g., A. fumigatus IcsA and yeast Dit1), (ii) two-domain isocyanide synthase-dioxygenases (ICS-DOXs) found in both bacteria (e.g., V. cholerae IsnAB) and eukaryotes (e.g., A. fumigatus IcsB and XanB), and (iii) the novel multidomain isocyanide synthase-NRPSs (ICS-NRPSs) found only in fungi (e.g., A. fumigatus CrmA). To further map the distribution of DOX and NRPS-like domains that accompany these ICSs, we reconstructed the phylogeny of dioxygenase domain (PF02668)-containing proteins ( Fig. 2B; see Fig. S4 in the supplemental material) and examined the taxonomic distribution of the NRPS-like (PF00501) domaincontaining proteins (Table S1). Unlike bacterial PF02668-type dioxygenases, which most often exist without an accompanying ICS domain, it is notable that all fungal PF02668type dioxygenase domain proteins exist as hybrid enzymes with ICSs ( Fig. 2B and Fig. S4). Closer examination of the fungal species that harbor ICS-NRPS proteins showed a striking enrichment of fungal pathogens of humans, insects, and plants (Table S1). Uncovering fungal copper-responsive ICS gene clusters. Isocyanides have long been recognized for their ability to form complexes with various transition metals (28). However, the effects of extra-and intracellular transition metal availability on the production of these compounds have not been explored. In a preliminary experiment to assess impact of metals on the expression of the four ICSs we identified in A. fumigatus, we found that several genes within the ICS3G BGC were expressed highly on undefined yeast extract-glucose (YG) medium but not on the standard Aspergillus defined glucose minimal medium (GMM) that is supplemented with transition metals, including zinc, manganese, iron, cobalt, copper, and molybdenum (data not shown).
To further investigate the role of transition metal availability for ICS3G expression, the wild-type (WT) strain of A. fumigatus (AF293) was grown on a series of GMM formulations, each lacking one transition metal. We found that a subset of four genes (crmA to crmD) within the ICS3G BGC are expressed when copper is not supplemented (copper depleted) and refer to these four genes as the crm cluster (Fig. 3A). Two crm genes are predicted to encode copper (crmD) and iron (crmC) transporters ( Fig. 1). To investigate the specificity of this transcriptional response, levels of expression of several verified copper-and iron-responsive genes were assessed. We found that expression patterns of the high-affinity copper transporter (ctrC) closely mimicked that of crmA to crmD (Fig. 3A). As expected, transcripts pertaining to high-affinity reductive iron uptake systems were upregulated in iron-depleted medium, whereas expression of crmA to crmD was not induced (Fig. 3A), demonstrating the specificity of the crmA to -D transcriptional response to copper.
To elucidate the regulatory network governing this copper-responsive transcriptional response, expression levels of crmA to -D were assessed in three copper-fist transcription factor mutants recently identified in A. fumigatus (29)(30)(31). MacA is important during copper starvation and induces a transcriptional network for copper uptake, AceA is crucial for initiating the detoxification program at toxic copper levels, and a CufA homologue plays a dual role in Cryptococcus neoformans (32), but its specific role or roles in A. fumigatus remain unclear. When grown in media lacking copper supplementation, we found that expression of crmA to -D is significantly reduced but not completely abolished in the ΔmacA mutant (Fig. 3B), suggesting a crucial role by MacA for inducing expression of crmA to -D during copper starvation. MacA also was critical for crm gene expression under copper-replete conditions, whereas loss of aceA or cufA had no to minimal impact on crm transcription.
We also found that a subset of the xan genes were regulated by both AceA and MacA. In contrast to crmA to -D, our data indicate that AceA is required for the expression of xanA to -C during copper starvation and both AceA and MacA are important for xan gene expression under copper-replete conditions (Fig. 3B). CufA loss did not impact xan gene expression. Finally, we did not observe expression of the two remaining ICS genes, icsA and icsB, under any of the tested conditions (data not shown). Taken together, our results demonstrate that both AceA and MacA play distinct roles upon governance of two ICS gene clusters, crm and xan, under both copper-depleted and copper-replete conditions.
The xan BGC encodes the xanthocillin biosynthetic pathway. Given that a previous study reported isolation of xanthocillin-like isocyanides from A. fumigatus (9), we asked whether any of the identified ICSs are involved in their biosynthesis. We Isocyanide Synthases in Aspergillus fumigatus ® constructed single and double ICS deletion mutants in the two BGCs expressed under our conditions: ICS3G (crmA) and ICS5G (xanB) (see Table S3 and Fig. S5 in the supplemental material). Given the transcriptional response to copper of crmA and xanB (Fig. 3), wild-type and deletion mutant strains were grown under both copper-depleted and copper-replete conditions. Metabolite extracts from the wild type and the three ICS deletion mutants were analyzed by liquid chromatography-high-resolution mass spectrometry (LC-HRMS), and the resulting metabolite profiles were compared using the XCMS data processing platform (33) (Fig. 4A to D).
We found that all four xanthocillin derivatives were abundantly produced in the wild type under copper-depleted conditions, whereas production was markedly reduced upon copper supplementation (Fig. 4A to D and Table 2), thus supporting the expression profile of the two synthases (Fig. 2). However, minute levels of compounds 7 to 10 could still be detected under copper-replete conditions (Fig. 4A to D and Table 2). Given that loss of XanB alone is sufficient to abrogate production of all four xanthocillin derivatives, we conclude that the ICS5G BGC encodes xanthocillin biosynthetic pathway in A. fumigatus. As there are no predicted transporters within the xan BGC, and given that both crmC and crmD are upregulated during copper starvation (Fig. 2), we created a strain deleted for both transporters (⌬crmC ⌬crmD) to assess whether these two putative metal transporters affect production of isocyanide derivatives in A. fumigatus (see Fig. S5 and Table S3 in the supplemental material). Despite being regulated in a copper-dependent manner, xanthocillin production in the ⌬crmC ⌬crmD mutant is similar to that of the wild-type strain grown under the same conditions (Fig. 4A to D and Table 2).
To further investigate regulation of the xan BGC, we overexpressed and deleted the sole transcription factor present in this cluster (XanC) to determine whether it controls expression of other cluster genes and formation of xan biosynthetic products (Fig. 1, Table S3, and Fig. S5). Supporting regulation of the xan BGC by XanC, expression of all xan genes (except xanD, encoding a putative protein with an uncharacterized domain [ Fig. 1]) is upregulated in the OE::xanC mutant (Fig. 5A). LC-HRMS analysis of the XanC overexpression mutant (OE::xanC) revealed increased abundance of all xanthocillin derivatives compared to the wild type under both copper-replete (5 M Cu) and -depleted conditions ( Fig. 5B and C and Table 2). Further, analysis of OE::xanC revealed two additional isonitrile-containing compounds, the sulfated xanthocillin derivative BU-4704 (compound 11) (Fig. 4E, Table 1, Fig. S6D, and Table S2D) (18) and xanthocillin X monomethyl ether (compound 12) (Fig. 4F and Table 1). Table 2 compares the production of compounds 7 to 12 in the OE::xanC mutant to those of the other mutants grown in copper-depleted medium, showing much higher production of all metabo-lites in the overexpression strain, with the exception of compound 12, the nonsulfated xanthocillin X monomethyl ether. Table 2 also validates the results from Fig. 4, showing the requirement of xanB for compounds 7 to 12. In contrast, deletion of xanC virtually eliminates all pathway metabolites ( Fig. 5D and E, Table 3, and Fig. S5).
Total production of xan biosynthetic products is elevated under all growth conditions in both the wild-type strain and OE::xanC mutant when A. fumigatus is grown on Extracted-ion chromatograms (EICs) corresponding to compounds 7 to 12 in the wild type grown with or without copper (orange and purple lines, respectively), in the ΔxanB, ΔcrmA, and ΔcrmCD mutants (blue, green, and red lines, respectively), the ΔxanB ΔcrmA double mutant under the copper-depleted condition (black lines), and the OE::xanC mutant with or without copper (black and red lines, respectively).

Isocyanide Synthases in Aspergillus fumigatus
® solid culture (Fig. 5D and E and Table 3). In-depth analysis of all intermediates showed that under liquid-submerged conditions, the production of dipeptides melanocins E (compound 9) and F (compound 10) is favored, whereas under solid-culture conditions, the isocyanide (compounds 11 and 12) and formamide (compound 8) moieties are favorably produced (Fig. 5B to E and Tables 2 and 3). The accumulation of isocyanide moieties from the xan biosynthetic pathway may provide the fungus with ecological benefits during asexual sporulation.
A model for xanthocillin biosynthesis in Aspergillus fumigatus. In conjunction with previous work on related enzymes in bacteria and yeast, our results suggest that XanB converts L-tyrosine to putative intermediate compound 14 in a two-step sequence analogous to bacterial IsnA-IsnB isocyanide synthase (Fig. 6) (5). Subsequent oxidative dimerization of compound 14 to form xanthocillin may involve XanG, a cytochrome P450 whose expression is coregulated with that of XanB. XanG is a homologue of the yeast enzyme Dit2, which catalyzes formation of N,N-bisformyl dityrosine, a spore wall component, which, similar to xanthocillin, is derived from dimerization of two tyrosine-derived moieties (Fig. 6) (36). As pointed out above, Dit1, the enzyme involved in the initial step of the yeast pathway to N,N-bisformyl dityrosine, shows high homology to other fungal ICSs and specifically the ICS domain of XanB, suggesting that the xanthocillin and N,N-bisformyl dityrosine biosynthetic pathways are closely related. The N-formyl moiety in compounds 7 to 9 is presumably introduced by XanA, a close homologue of a known isocyanide hydratase, which may act as part of isocyanide detoxification (37). The methyl moiety in six xanthocillin derivatives is likely introduced by the putative methyltransferase XanE. Finally, both melanocins E (compound 9) and F (compound 10) appear to be derived from the cyclization of compound 8 (Fig. 6).

DISCUSSION
Many naturally occurring isocyanides have bioactivities of ecologic, economical, and pharmaceutical importance due to their structurally diverse carbon scaffolds and a highly reactive isocyanide moiety capable of coordinating transition metals. Despite their diverse biological activities, isocyanide biosynthesis had previously been studied only in bacteria. Our investigation of ICSs in the opportunistic human fungal pathogen  A. fumigatus revealed the fungal xanthocillin biosynthetic pathway and hints at diverse functions of ICS homologues in eukaryotes. Based on the phylogeny of the ICS domain (PF05010), we found that A. fumigatus harbors all three variations of ICS domain proteins ( Fig. 1 and 2). ICS homologues appear to be widely distributed in both bacteria and fungi, with outlier representation in oomycetes, plants, and metazoans (Fig. 2). Fungal ICSs do not appear to have been recently horizontally acquired from bacteria, are evolutionarily arranged into several distinct fungal clades, and occur particularly concentrated within the phylum Ascomycota (Fig. 2). Significantly, our examination of the ICS phylogeny revealed a recent evolutionary expansion of the novel ICS-NRPS hybrid enzymes in many important fungal pathogens and highlighted the presence of ICS homologues in a yeast taxon (Saccharomycotina) not normally known to harbor extensive secondary metabolism capabilities, including the human-pathogenic Candida spp. (Fig. 2A, Table S1, and Fig. S3). A detailed examination of the xan gene cluster architecture showed conservation of five xan genes (xanA, xanB, xanC, xanD, and xanG) in both Penicillium chrysogenum and Penicillium expansum (Fig. 7). P. expansum has been reported to produce the isocyanide xanthocillin X (38). However, only xanA and xanB are conserved in Penicillium oxalicum (Fig. 7). Conservation of flanking genes but not the xan BGC in   (Fig. 7). Yeast ICS homologues represent a family of proteins named Dit1, which are involved in the production of the sexual spore wall component N,N-bisformyl dityrosine in S. cerevisiae (27) and likely in C. albicans as well (39,40). However, the specific enzymatic function of this protein, including its enzymatic product, remains unknown. In both S. cerevisiae and C. albicans, the dit1 gene is transcribed divergently from dit2, encoding a cytochrome P450 monooxygenase, which is assumed to catalyze the formation of N,N-bisformyl dityrosine from the product of Dit1 catalysis (Fig. 6) (27). Dit1 and Dit2 are homologues of XanB and XanG in A. fumigatus, respectively. Given that the putative mechanism of N,N-bisformyl dityrosine formation is strikingly similar to that of xanthocillin biosynthesis, we propose the unknown product of Dit1 catalysis in S. cerevisiae to be a tyrosine isocyanide precursor (compound 13) (Fig. 6).
Our results demonstrate that in A. fumigatus, XanB is the dedicated synthase for production of the copper-responsive isocyanides and their derivatives, and we suggest that the Dit2-like protein XanG catalyzes oxidative dimerization, following isocyanide formation via XanB. We showed that both copper-fist transcription factors (AceA and MacA) are involved in governing the copper-responsive profile of the xan and crm BGCs. Although the crm BGC is similarly regulated by copper availability, we find no direct involvement of this BGC in the production of compounds 7 to 12. Given that CrmA is a novel type of ICS-NRPS hybrid enzyme within the ICS3G-BGC found to be enriched in many fungal pathogens of plants, insects, and humans, our ongoing work aims to elucidate the activity of this enzyme and identify its biosynthetic product(s), to further define the copper-responsive regulation of its gene cluster, and ultimately to query their role or roles in the ecologic fitness or pathogenicity potential of A. fumigatus and other pathogenic fungi. The involvement of this family of ICSs in fungi, on one hand, to produce an important cell wall dipeptide (dityrosine) in yeast, and on the other hand, to produce a potential defensive molecule (xanthocillin) in filamentous mold makes for their interesting position at the interface of both primary and secondary metabolism.
The discovery of diverse ICSs in fungi revealed a novel, unexplored aspect of fungal secondary metabolism. Whereas iron-responsive secondary metabolism in fungi is well understood, our characterization of xanthocillin biosynthesis and regulation of the synthases involved in its production in A. fumigatus offers the first insight into copperresponsive secondary metabolism in fungi, possibly related to copper chelation processes, as has been found for a few other natural products, such as yersiniabactin and methanobactins (41,42). Finally, it should be noted that two of the identified ICS BGCs were "invisible" to current secondary metabolite prediction algorithms, including antiSMASH (26) and SMURF (25) because of the lack of precedent for eukaryotic ICSs and absence of canonical class-defining secondary-metabolite-producing synthases (e.g., nonribosomal peptide synthetase, polyketide synthase, terpene cyclase, prenyltransferase, etc.). Revision of such algorithms will further expand the power of bioinformatically driven natural product mining. Putative Fatty Acid Elongase P c x a n A P c x a n B P c x a n C P c x a n D P c x a n G x a n A x a n B x a n C x a n D x a n E x a n F x a n G P e x x a n A P e x x a n B P e x x a n C P e x x a n D P e x x a n G

Penicillium oxalicum
P o x x a n A P o x x a n B

MATERIALS AND METHODS
Fungal growth and culture conditions. All A. fumigatus strains used in this study are listed in Table S3. Strains were maintained as glycerol stocks and activated on solid glucose minimal medium (GMM) at 37°C (37). Growth medium was supplemented with 5.0 mM uridine and uracil and 5.7 mM arginine for pyrG and argB auxotrophs, respectively. For isolation of genomic DNA (gDNA) for PCR and Southern blotting, 10 ml of liquid minimal medium (43) with yeast extract was inoculated with spores from solid medium and grown overnight at 37°C. For isolation of the OE::xanC and wild-type AF293 RNA, 50 ml of GMM was inoculated (1.0 ϫ 10 6 spores per ml), and cells were grown for~12 h at 37°C with shaking at 200 rpm. For cDNA synthesis of crmA, A. fumigatus AF293 was inoculated at 1.0 ϫ 10 6 spores per ml into yeast extract-glucose (YG) medium for the inducing condition and YG medium plus trace elements for the noninducing condition and cultured at 37°C for 24 h. For ultrahigh-performance liquid chromatography (UHPLC)-HRMS and NMR analysis, strains were inoculated (1.0 ϫ 10 6 spores per ml) into 50 ml GMM with (5 M) and without copper supplementation in a 125-ml Erlenmeyer flask at 37°C with shaking at 200 rpm for 120 h. For metabolite analysis under solid conditions, 1 ϫ 10 8 total spores were plated onto GMM solid plates with (5 M) and without copper. For copper-starved growth, conidia used for inoculation were harvested from copper-depleted GMM. For copper-replete growth, conidia used for inoculation were harvested from GMM containing 5 M supplemented copper.
Mutant construction. The mutants used in this study (Table S3) were created using the double-joint PCR (DJ-PCR) method (44). Genomic DNA and RNA were isolated from A. fumigatus AF293 using standard procedures. Construction of DJ-PCR products, protoplast production, and transformation were carried out as previously described (44). Briefly, primers were designed to amplify approximately 1,000-bp flanks with 20 bp of overlap of the selection marker-containing plasmid using SeqBuilder within the Laser-gene12 suite (DNASTAR, Madison, WI). For deletion strains, plasmid pJW24 (45) or pJMP4 (46) was used to amplify the A. parasiticus pyrG or A. fumigatus argB genes, respectively. For the xanC overexpression strain (OE::xanC mutant), plasmid pJMP9.1 (47) was used to amplify the A. parasiticus pyrG gene fused to an A. nidulans gpdA constitutively active promoter. Both pyrG Ϫ (TFYL80) and argB Ϫ (TFYL84) mutants were generated by complementing the pyrG-argB Ϫ double auxotroph (TFYL45) with A. fumigatus argB (pJMP4) and A. fumigatus pyrG (pKJA12), respectively. The ⌬crmA pyrG Ϫ mutant (TFYL90) was generated from TFYL45. The ⌬crmA mutant (TFYL93) was generated from TFYL84. The ⌬xanB mutant (TFYL105) was generated from TFYL80.1. The ⌬crmA ⌬xanB double mutant was generated from TFYL90. The OE::xanC mutant was generated from TFYL80. Transformants were screened for proper integration of the construct and loss of the gene of interest via PCR. Single integration of the transformation cassette was verified by Southern analysis (Fig. S5). Expression of xanC in the OE::xanC mutant was confirmed via Northern analysis. For all auxotrophic mutants, maintenance of the mutant allele(s) was confirmed via PCR after complementation to prototrophy.
cDNA synthesis and cloning of crmA. Fungal mycelia were harvested from cells under both inducing and noninducing conditions and lyophilized. Total RNA was extracted from the lyophilized mycelia using TRIzol according to the manufacturer's protocol. Ten micrograms of total RNA was digested with DNase I and subjected to cDNA synthesis using the SuperScript III first-strand reverse transcriptase system according to the manufacturer's protocol. The full-length crmA genes according to the new prediction (Fig. S1) were amplified and cloned into bacterial expression vector pTEV5 (48) to give rise to pFYL15.
Phylogenetic analysis. To identify isocyanide synthases in A. fumigatus, a PSI-BLAST search using amino acid sequences of the bacterial isocyanide synthase from P. aeruginosa, PvcA, was queried against the genome of A. fumigatus AF293. To build the phylogenetic trees, the regions corresponding to isocyanide synthase (Pfam domain PF05141), Fe 2ϩ ␣-ketoglutarate-dependent dioxygenase (Pfam domain PF02668) in the ICSs of Aspergillus fumigatus AF293, CrmA, and XanB, were queried against Ensembl Genomes using phmmer, a member of the HMMER3 software suite (49) (web server accessed 15 July 2016). Significant hits were downloaded and filtered based on length and sequence composition and to reduce redundancy in the data set using a custom perl script as well as IQ-TREE (50) (Table S1). Following this filtering step, regions corresponding to each domain of interest were aligned with MAFFT v7.023b using the G-INS-I strategy (51), and the topologies were inferred using maximum likelihood (ML) as implemented in RAxML version 8.0.25 (52) using a PROTGAMMALG substitution model (automatically determined to be the best model within RAxML) and rapid bootstrapping (100 replications). All alignments and trees are available for download on the figshare data repository (https://doi.org/10.6084/ m9.figshare.4721116.v1). The phmmer-identified sequences were scanned for significant hits to additional Pfam domains using an hmmsearch E value inclusion threshold of 0.001 (Table S1).
Analytic methods and equipment overview. (i) NMR spectroscopy. NMR spectroscopy was performed on a Bruker Avance III HD (800-MHz 1 H reference frequency, 201 MHz for 13 C) equipped with a 5-mm CPTCL 1 H-13 C/ 15 N cryo probe. Non-gradient phase-cycled double quantum-filtered correlation spectroscopy (DQF-COSY) spectra were acquired using the following parameters: 0.6-s acquisition time; 400 to 600 complex increments, and 8, 16, or 32 scans per increment. Heteronuclear single quantum coherence (HSQC) and heteronuclear multiple bond correlation (HMBC) spectra were acquired with the following parameters: 0.25-s acquisition time, 200 to 500 complex increments, and 8 to 64 scans per increment. 1 H, 13 C-HMBC spectra were optimized for J H,C ϭ 6 Hz. HSQC spectra were acquired with or without decoupling. NMR spectra were processed and baseline corrected using MestreLabs MNOVA software packages.
(ii) MS. Ultrahigh-performance liquid chromatography-high-resolution MS (UHPLC-HRMS) was performed on a Thermo Scientific-Dionex Ultimate 3000 UHPLC system equipped with a diode array detector and connected to a Thermo Scientific Q Exactive Orbitrap operated in electrospray ionizationpositive (ESI ϩ ) or -negative (ESI Ϫ ) mode.
Metabolite extraction and LC-MS analysis. Liquid fungal cultures (50 ml) including fungal tissue and medium were frozen using a dry ice-acetone bath and lyophilized. The lyophilized residues were extracted with 20 ml of ethyl acetate-methanol (9:1) for 1.5 h with vigorous stirring. Extracts were filtered over cotton, evaporated to dryness, and stored in 4-ml vials. Crude extracts were suspended in 0.5 ml of methanol and centrifuged to remove insoluble materials, and the supernatant was subjected to UHPLC-HRMS analysis. An Agilent Zorbax RRHD Eclipse XDB-C 18 column (2.1 by 100 mm, 1.8-m particle diameter) was used with acetonitrile (organic phase) and 0.1% formic acid in water (aqueous phase) as solvents at a flow rate of 0.5 ml/min. A solvent gradient scheme was used, starting at 2% organic for 1 min, followed by a linear increase to 100% organic over 14 min, holding at 100% organic for 2.5 min, decreasing back to 2% organic for 0.1 min, and holding at 2% organic for the final 1.4 min, for a total of 18 min.
Chromatographic purification of compounds 7, 9, 10, and 11. Liquid fungal cultures (1 liter) including fungal tissue and medium were frozen using a dry ice-acetone bath and lyophilized. The combined lyophilized residues were extracted with 500 ml of ethyl acetate-methanol (9:1) for 3.5 h with vigorous stirring. Extracts were filtered over cotton, evaporated to dryness, and stored in 8-ml vials. Crude extracts were fractionated via semipreparative HPLC using an Agilent XDB C 8 column (25 cm by 10 mm, 5-m particle diameter) with acetonitrile (organic phase) and 0.1% acetic acid in water (aqueous phase) as solvents at a flow rate of 3.2 ml/min. A solvent gradient scheme was used, starting at 5% organic for 3 min, followed by a linear increase to 100% organic over 27 min, holding at 100% organic for 5 min, decreasing back to 5% organic for 0.1 min, and holding at 5% organic for the final 4.9 min, for a total of 40 min. Further purification of fractions containing compounds 7, 9, 10, and 11 was accomplished by semipreparative HPLC using an Agilent XDB C 8 column (25 cm by 10 mm, 5-m particle diameter) with acetonitrile (organic phase) and 0.1 M (pH 8.0) ammonium acetate in water (aqueous phase) as solvents at a flow rate of 3.2 ml/min with same gradient scheme described above.