Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Latest Articles
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • Topics
    • Applied and Environmental Science
    • Clinical Science and Epidemiology
    • Ecological and Evolutionary Science
    • Host-Microbe Biology
    • Molecular Biology and Physiology
    • Therapeutics and Prevention
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About mBio
    • Editor in Chief
    • Board of Editors
    • AAM Fellows
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
mBio
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Latest Articles
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • Topics
    • Applied and Environmental Science
    • Clinical Science and Epidemiology
    • Ecological and Evolutionary Science
    • Host-Microbe Biology
    • Molecular Biology and Physiology
    • Therapeutics and Prevention
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About mBio
    • Editor in Chief
    • Board of Editors
    • AAM Fellows
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
Research Article

Heterogeneous and Flexible Transmission of mcr-1 in Hospital-Associated Escherichia coli

Yingbo Shen, Zuowei Wu, Yang Wang, Rong Zhang, Hong-Wei Zhou, Shaolin Wang, Lei Lei, Mei Li, Jiachang Cai, Jonathan Tyrrell, Guo-Bao Tian, Congming Wu, Qijing Zhang, Jianzhong Shen, Timothy R. Walsh, Zhangqi Shen
Eric A. Johnson, Editor
Yingbo Shen
aBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
bBeijing Key Laboratory of Detection Technology for Animal-Derived Food Safety and Beijing Laboratory for Food Quality and Safety, Beijing, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zuowei Wu
cCollege of Veterinary Medicine, Iowa State University, Ames, Iowa, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yang Wang
aBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
bBeijing Key Laboratory of Detection Technology for Animal-Derived Food Safety and Beijing Laboratory for Food Quality and Safety, Beijing, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rong Zhang
dSecond Affiliated Hospital of Zhejiang University, Zhejiang University, Hangzhou, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hong-Wei Zhou
dSecond Affiliated Hospital of Zhejiang University, Zhejiang University, Hangzhou, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shaolin Wang
aBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Shaolin Wang
Lei Lei
aBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mei Li
aBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
eDepartment of Medical Microbiology and Infectious Disease, Institute of Infection & Immunity, Heath Park Hospital, Cardiff, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jiachang Cai
dSecond Affiliated Hospital of Zhejiang University, Zhejiang University, Hangzhou, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jonathan Tyrrell
eDepartment of Medical Microbiology and Infectious Disease, Institute of Infection & Immunity, Heath Park Hospital, Cardiff, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Guo-Bao Tian
fZhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Congming Wu
aBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
bBeijing Key Laboratory of Detection Technology for Animal-Derived Food Safety and Beijing Laboratory for Food Quality and Safety, Beijing, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Qijing Zhang
cCollege of Veterinary Medicine, Iowa State University, Ames, Iowa, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jianzhong Shen
aBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
bBeijing Key Laboratory of Detection Technology for Animal-Derived Food Safety and Beijing Laboratory for Food Quality and Safety, Beijing, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Timothy R. Walsh
eDepartment of Medical Microbiology and Infectious Disease, Institute of Infection & Immunity, Heath Park Hospital, Cardiff, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zhangqi Shen
aBeijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing, China
bBeijing Key Laboratory of Detection Technology for Animal-Derived Food Safety and Beijing Laboratory for Food Quality and Safety, Beijing, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eric A. Johnson
University of Wisconsin—Madison
Roles: Editor
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/mBio.00943-18
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Supplemental Material
  • FIG 1 
    • Open in new tab
    • Download powerpoint
    FIG 1 

    Genomic analysis of mcr-1-carrying E. coli isolates in a single hospital system in Zhejiang Province, China. A maximum-likelihood phylogenetic tree was constructed using the core genome SNPs and midpoint rooted. Sources of the isolates are indicated by different colors for strain identification (ID) plus MLST (red, infectious sample of inpatient; green, feces of inpatient; black, feces of healthy volunteer). E. coli phylogroups are denoted by colored strips, and the branches of the tree are colored in correspondence to the coloring of the strips. The location of mcr-1 on a plasmid or chromosome (dark red) and the presence or absence of virulence genes (green) and antibiotic resistance genes (orange) are denoted by filled and empty squares, respectively. Only categories of the virulence genes and 9 clinically important antibiotic resistance genes are shown. Details of the genes in each category are given in Table S4 and S5.

  • FIG 2 
    • Open in new tab
    • Download powerpoint
    FIG 2 

    Schematic depiction of the prevalence of mcr-1 in various types of plasmids or on the chromosomes. The types of plasmids potentially carrying the mcr-1 gene were derived from the mcr-1-carrying contigs of each isolate. A colored circle represents a reference mcr-1-carrying plasmid of each type or the complete plasmid determined by SMRT sequencing in this study, and the size of the circle is proportional to that of the reference plasmid. Ten mcr-1-carrying plasmids whose replicons were not in the same contigs as mcr-1 are grouped in one box (Inc). mcr-1 is chromosome-borne in 7 isolates and plasmid-borne in 73 isolates (central pie chart). IncI2 and IncX4 are the major types of mcr-1-carrying plasmids. Isolates ZJ859 and ZJ1432 contained two mcr-1-carrying plasmids each. Thus, the total number of mcr-1-carrying plasmids is 75 in this study.

    • Open in new tab
    • Download powerpoint
    • Open in new tab
    • Download powerpoint
    FIG 3 

    The diverse genetic environments of mcr-1 in 80 E. coli isolates. In panels A to G, red fonts indicate isolates from inpatients with infections; green fonts denote isolates from feces of inpatients; and black fonts depict isolates from feces of healthy volunteers. APEC, avian pathogenic E. coli; ORF, open reading frame.

Supplemental Material

  • Figures
  • TABLE S1 

    The E. coli isolates in this study and their information from sequencing analysis. Download TABLE S1, XLSX file, 0.04 MB.

    Copyright © 2018 Shen et al.

    This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

  • TABLE S2 

    Clinical information on 80 E. coli isolates with the mcr-1 gene and the MICs of multiple antimicrobial agents. Download TABLE S2, XLSX file, 0.05 MB.

    Copyright © 2018 Shen et al.

    This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

  • TABLE S3 

    The assembly stats for the PacBio genomes. Download TABLE S3, XLSX file, 0.03 MB.

    Copyright © 2018 Shen et al.

    This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

  • TABLE S4 

    Characterization of virulence genes in 80 mcr-1-carrying E. coli isolates. Download TABLE S4, XLSX file, 0.1 MB.

    Copyright © 2018 Shen et al.

    This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

  • TABLE S5 

    Characterization of resistance genes in 80 mcr-1-carrying E. coli isolates. Download TABLE S5, XLSX file, 0.1 MB.

    Copyright © 2018 Shen et al.

    This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

  • TABLE S6 

    Allelic variations of seven mcr-1-carrying isolates. Download TABLE S6, XLSX file, 0.03 MB.

    Copyright © 2018 Shen et al.

    This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

PreviousNext
Back to top
Download PDF
Citation Tools
Heterogeneous and Flexible Transmission of mcr-1 in Hospital-Associated Escherichia coli
Yingbo Shen, Zuowei Wu, Yang Wang, Rong Zhang, Hong-Wei Zhou, Shaolin Wang, Lei Lei, Mei Li, Jiachang Cai, Jonathan Tyrrell, Guo-Bao Tian, Congming Wu, Qijing Zhang, Jianzhong Shen, Timothy R. Walsh, Zhangqi Shen
mBio Jul 2018, 9 (4) e00943-18; DOI: 10.1128/mBio.00943-18

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this mBio article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Heterogeneous and Flexible Transmission of mcr-1 in Hospital-Associated Escherichia coli
(Your Name) has forwarded a page to you from mBio
(Your Name) thought you would be interested in this article in mBio.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Heterogeneous and Flexible Transmission of mcr-1 in Hospital-Associated Escherichia coli
Yingbo Shen, Zuowei Wu, Yang Wang, Rong Zhang, Hong-Wei Zhou, Shaolin Wang, Lei Lei, Mei Li, Jiachang Cai, Jonathan Tyrrell, Guo-Bao Tian, Congming Wu, Qijing Zhang, Jianzhong Shen, Timothy R. Walsh, Zhangqi Shen
mBio Jul 2018, 9 (4) e00943-18; DOI: 10.1128/mBio.00943-18
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • INTRODUCTION
    • RESULTS
    • DISCUSSION
    • MATERIALS AND METHODS
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

E. coli
genetic diversity
MCR-1
population genomics
transmission

Related Articles

Cited By...

About

  • About mBio
  • Editor in Chief
  • Board of Editors
  • AAM Fellows
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Author Warranty
  • Article Types
  • Ethics
  • Contact Us

Follow #mBio

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Online ISSN: 2150-7511