Article Figures & Data
Figures
- FIG 1
Pulsed-field gel electrophoresis for the accessory chromosomes of Z. tritici. Pictures of three pulsed-field gel electrophoresis gels for all strains used in this study are shown. Size marker, Saccharomyces cerevisiae. White arrows indicate missing chromosome bands. Absence of chromosome 14 is observed for strains Zt260, Zt295, and Zt273. Absence of chromosome 15 is observed for strains Zt291, Zt248, and Zt294; absence of chromosome 16 or 17 or 18 can be inferred for strains Zt251, Zt270, Zt271, Zt299, Zt265, Zt262, Zt272, and Zt290. Absence of chromosome 19 is observed for strain Zt278; absence of chromosome 20 is observed for strains Zt266 and Zt279; and absence of chromosome 21 is observed for strains Zt267, Zt269, and Zt293.
- FIG 2
Genome-wide map of Illumina read coverage of the IPO323 progenitor strain and the aneuploid strains. The absence of reads mapped to the deleted accessory chromosomes confirms chromosome loss in the respective strains. Increased coverage of chromosome 14 in strain Zt262 indicates chromosome duplication, while the lower coverage of chromosome 14 in strain Zt266, on the other hand, suggests a mix of cells with and without this chromosome.
- FIG 3
Pycnidia density of the IPO323 progenitor and the aneuploid strains produced at 21 dpi in different wheat cultivars. Box plots are shown for pycnidia density data from wheat cultivars (cv) (A) Obelisk, (B) Runal, (C) Titlis, and (D) Riband. Statistical significance, inferred through an ANOVA and a subsequent post hoc Tukey’s HSD test comparing the aneuploid strains to the IPO323 reference, is indicated as follows: *, P < 0.05; **, P < 0.005; ***, P < 0.0005. Data shown are based on results from (A) three, (B and D) four, or (D) two independent experiments.
- FIG 4
Timing of the switch to necrotrophy of the IPO323 progenitor and the aneuploid strains. (A to C) Boxplots depicting the day postinfection at which the first necrotic symptoms appeared on the leaf surface for cultivars (A) Obelisk, (B) Runal, and (C) Titlis. Data used in the analyses were pooled from results from 3 independent experiments. Statistical significance, inferred through an ANOVA and subsequent post hoc Tukey’s HSD test comparing the aneuploid strains to the IPO323 reference, is indicated as follows: *, P < 0.05; **, P < 0.005; ***, P < 0.0005.
- FIG 5
The timing of switch to necrotrophy influences the number of pycnidia produced. (A to C) Boxplots of the pycnidia density on leaves at 21 dpi grouped according to the day (postinfection) when the first necrotic symptoms appeared on the respective leaf for cultivars (A) Obelisk, (B) Runal, and (C) Titlis. Data for all tested Z. tritici strains were pooled for each of the cultivars. The shapes of the curves as tested by the interaction of the factor cultivar and the switch to necrotrophy proved significantly different for the pairwise comparisons of Obelisk and Titlis (P = 7.0 × 10−6).
Tables
- TABLE 1
Absolute and relative frequencies of unique chromosomal loss events in IPO323 in the presence and absence of carbendazim
Accessory
chromosomeNo. of chromosomal losses With
carbendazimWithout
carbendazimTotal 14 2 2 4 15 12 1 13 16 5 0 5 17 3 1 4 18 4 0 4 19 0 0 0 20 2 0 2 21 5 0 5 - TABLE 2
Absolute frequencies of novel SNPs and small indels for the aneuploid strains not present in the IPO323 progenitor strain
Strain Chromosomal
statusNo. of novel SNPs/indels
(∑ = 51)No. of novel SNPs/indels
in coding sequences (∑ = 9)Zt260 ΔChr14 4 0 Zt295 ΔChr14 6 3 Zt291 ΔChr15 3 2 Zt248 ΔChr15 2 1 Zt251 ΔChr16 2 0 Zt270 ΔChr16 1 0 Zt271 ΔChr17 7 1 Zt299 ΔChr17 8 0 Zt262 ΔChr18 6 0 Zt272 ΔChr18 1 1 Zt290 ΔChr18 1 0 Zt278 ΔChr19 2 0 Zt266 ΔChr20 0 0 Zt279 ΔChr20 2 0 Zt267 ΔChr21 4 0 Zt269 ΔChr21 2 1
FIG S1
Gel electrophoresis for PCR karyotyping of strains used in this study of (A) multiplexed PCR targeting the subtelomeric region of chromosomes 14 to 21 (B to I) by PCR (B) along chromosome 14, (C) along chromosome 15, (D) along chromosome 16, (E) along chromosome 17, (F) along chromosome 18, (G) along chromosome 19, (H) along chromosome 20, and (I) along chromosome 21. Download FIG S1, PDF file, 0.7 MB.
Copyright © 2017 Habig et al.
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FIG S2
Pulsed-field gel electrophoresis for midsize core chromosomes of Z. tritici. Pictures of three pulsed-field gel electrophoresis gels for all strains used in this study are shown. Size marker, Hansenula wingei chromosomes. Download FIG S2, PDF file, 0.2 MB.
Copyright © 2017 Habig et al.
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FIG S3
Pulsed-field gel electrophoresis for large core chromosomes of Z. tritici. Pictures of three pulsed-field gel electrophoresis gels for all strains used in this study are shown. Size marker, Schizosaccharomyces pombe chromosomes. Download FIG S3, PDF file, 0.2 MB.
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TABLE S1
Summary of next-generation sequencing data and read mapping to the IPO323 reference genome. Download TABLE S1, XLSX file, 0.01 MB.
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FIG S4
Illustration of the locations of all single nucleotide polymorphisms (SNPs) and indels identified in all sequenced strains. Data represent genome-wide distribution of SNPs across the 13 core and 8 accessory chromosomes of reference strain IPO323. Download FIG S4, PDF file, 0.1 MB.
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TABLE S2
Summary of identified SNP and indels in the resequenced genomes of the aneuploid strains. Download TABLE S2, XLSX file, 0.01 MB.
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FIG S5
In vitro phenotypes of IPO323 and aneuploid strains. In vitro growth phenotypes were assessed for the chromosome deletion mutants. Cells were grown using different conditions and on different media, including temperature stress and cell wall stress agents. Download FIG S5, PDF file, 0.3 MB.
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TABLE S3
List of all primers used in this study. Download TABLE S3, XLSX file, 0.01 MB.
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TEXT S1
Protocol of read processing, reference assembly, and SNP calling. Download TEXT S1, PDF file, 0.3 MB.
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TABLE S4
Summary of in planta results. Download TABLE S4, XLSX file, 0.3 MB.
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This content is distributed under the terms of the Creative Commons Attribution 4.0 International license .
