Article Figures & Data
Figures
- FIG 1
Chemical and microbial ecology analysis of sediments and water from the site F aquifer. (A) Sediment (Sed) arsenic and iron concentration and reflectance curve (blue, red, and green) along the sediment depths with the presence of dissimilatory arsenic-respiring bacteria (brown) and iron-reducing members of the Geobacteraceae (lines). (B) As(V), As(III), and AS2S3 species in the sediment (XANES) at site F. (C) Arsenic X-ray absorption near the edge structure (XANES) spectra along various depths of sediments. (D) Fraction of the Fe phases in relation to the Fe minerals along various depths. (E) As, Fe concentration (blue and green) in wells (aqueous phase), and groundwater tritium age (red). (F) 16S rRNA gene analysis for the microbial communities. Bar diagrams indicate the percentage of each class of the bacterial kingdom.
- FIG 2
Chemical and microbial ecology analysis of sediments and water from the site B aquifers. (A) Sediment (Sed) arsenic and iron concentration and reflectance curve (blue, red, and green) along the sediment depths with the presence of dissimilatory arsenic-reducing bacteria (brown) and iron-reducing members of the Geobacteraceae (lines). (B) As(V), As(III), and AS2S3 species in the sediment (XANES) at site B. (C) Arsenic X-ray absorption near the edge structure (XANES) spectra along various depths of sediments. (D) Fraction of Fe phases in relation to the Fe minerals along various depths. (E) As, Fe concentration (blue and green) in wells (aqueous phase), and groundwater tritium age (red). (F) 16S rRNA gene analysis for the microbial communities. The bar diagram indicates the percentage of each class of the bacterial kingdom.
- FIG 3
Top Spearman rank correlation (P = 0.01) plots relating microbial communities to mineral species. (A, D, and G) As(III) correlation with the microbial communities at site B, site F, and at combined sites. (B, E, and H) Fe(III) correlation with the microbial communities at site B, site F, and at combined sites. (C, F, and I) Aqueous As correlation with the microbial communities.
Supplemental Material
TABLE S1
Chemical and molecular ecology analysis of sediments and water from the site F aquifer. Download TABLE S1, DOCX file, 0.1 MB.
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TABLE S2
Chemical and molecular ecology analysis of sediments and water from the site B aquifer. Download TABLE S2, DOCX file, 0.1 MB.
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FIG S1
PCR confirmation of functional genes. The positive control (+ve) for the arrA gene is Shewanella sp. strain ANA-3, whereas the positive control for Geobacteraceae specific 16S rRNA gene and dsr gene is Geobacter sulfurreducens. The sizes of the amplified products are 625 and 1,900 bp, respectively. Download FIG S1, TIF file, 1.3 MB.
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TABLE S3
Correlations between mineralogy and the molecular data. Download TABLE S3, DOCX file, 0.1 MB.
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FIG S2
Top 9 correlation of bacteria with Fe and As at site F. Download FIG S2, TIF file, 2.3 MB.
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FIG S3
Top 9 correlation of bacteria with arsenic at site F. Download FIG S3, TIF file, 1.2 MB.
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FIG S4
Top 9 correlation of bacteria with Fe and As at site B. Download FIG S4, TIF file, 1.4 MB.
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FIG S5
Top 9 correlation of bacteria with arsenic at site B. Download FIG S5, TIF file, 1.1 MB.
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FIG S6
Top 9 correlation of bacteria with Fe and As at sites F and B. Download FIG S6, TIF file, 1.3 MB.
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FIG S7
Top 9 correlation of bacteria with arsenic at sites F and B. Download FIG S7, TIF file, 1.2 MB.
Copyright © 2017 Gnanaprakasam et al.
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