Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Latest Articles
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • Topics
    • Applied and Environmental Science
    • Clinical Science and Epidemiology
    • Ecological and Evolutionary Science
    • Host-Microbe Biology
    • Molecular Biology and Physiology
    • Therapeutics and Prevention
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About mBio
    • Editor in Chief
    • Board of Editors
    • AAM Fellows
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
mBio
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Latest Articles
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • Topics
    • Applied and Environmental Science
    • Clinical Science and Epidemiology
    • Ecological and Evolutionary Science
    • Host-Microbe Biology
    • Molecular Biology and Physiology
    • Therapeutics and Prevention
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About mBio
    • Editor in Chief
    • Board of Editors
    • AAM Fellows
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
Commentary

Emergence of Epidemic Zika Virus Transmission and Congenital Zika Syndrome: Are Recently Evolved Traits to Blame?

Scott C. Weaver
Scott C. Weaver
Institute for Human Infections and Immunity and Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/mBio.02063-16
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

ABSTRACT

The mechanisms responsible for the dramatic emergence of Zika virus (ZIKV), accompanied by congenital Zika syndrome and Guillain-Barré syndrome (GBS), remain unclear. However, two hypotheses are prominent: (i) evolution for enhanced urban transmission via adaptation to mosquito vectors, or for enhanced human infection to increase amplification, or (ii) the stochastic introduction of ZIKV into large, naive human populations in regions with abundant Aedes aegypti populations, leading to enough rare, severe infection outcomes for their first recognition. Advances in animal models for human infection combined with improvements in serodiagnostics, better surveillance, and reverse genetic approaches should provide more conclusive evidence of whether mosquito transmission or human pathogenesis changed coincidentally with emergence in the South Pacific and the Americas. Ultimately, understanding the mechanisms of epidemic ZIKV emergence, and its associated syndromes, is critical to predict future risks as well as to target surveillance and control measures in key locations.

The views expressed in this Commentary do not necessarily reflect the views of this journal or of ASM.

COMMENTARY

Zika virus (ZIKV) has emerged over the past 10 years to cause outbreaks of human infection in the Pacific Islands followed by the Americas, where it is poised to cause millions of infections. Most of these infections are asymptomatic, and the majority of symptomatic cases are mild with rash, conjunctivitis, joint pains, and fever. However, infection can occasionally lead to Guillain-Barré syndrome (GBS), a paralytic disease of the peripheral nervous system, or a wide range of congenital defects and malformations, including microcephaly, when a pregnant woman is infected (1). Because these severe outcomes of ZIKV infection as well as the magnitude of the outbreaks in French Polynesia and the Americas are unprecedented, explanations for the recent emergence have mainly focused on two hypotheses.

(i) Adaptive evolution.In a recent article, Pettersson et al. (2) addressed the hypothesis that the recent emergence of ZIKV epidemic transmission and congenital Zika syndrome is associated with genetic changes in the virus that accompanied its spread to the South Pacific and subsequently to Latin America.

Like dengue virus (DENV) and chikungunya virus (CHIKV), ZIKV appears to have originated in zoonotic transmission cycles involving nonhuman primates and arboreal mosquito vectors (3); also like CHIKV, ZIKV evolved and was first discovered in sub-Saharan Africa (Fig. 1). The divergence of the African and Asian lineages (4) probably occurred many decades ago, with the spread beyond Southeast Asia to Pacific islands first detected in 2007 in Yap (5). Pettersson et al. (2) hypothesize that, also like CHIKV (6), ZIKV may have recently undergone adaptive evolution for enhanced transmission by urban mosquito vectors, possibly accompanied by increased human pathogenesis. Using phylogenetic analyses, amino acid changes associated with the emergence of ZIKV from Africa into Asia since the mid-20th century were assigned to tree branches representing different phases of virus movement. These analyses logically emphasize those amino acid changes accompanying the spread into French Polynesia, where the first association between ZIKV infection and Guillain-Barré syndrome (GBS) (7), as well as congenital microcephaly (8), occurred.

FIG 1 
  • Open in new tab
  • Download powerpoint
FIG 1 

Map of ZIKV evolution in sub-Saharan Africa, spread to Asia, and introductions into Yap Island, the South Pacific, and the Americas inferred from phylogenetic data. Adapted from reference 3 with permission.

Adaptive evolution for enhanced infection of vectors or vertebrate hosts by arboviruses is expected to be mediated mainly by amino acid substitutions in viral proteins. Several substitutions in the ZIKV premembrane protein previously discussed as potentially altering its structure (9) were found to occur on tree branches not leading to the French Polynesian strains, disputing their putative role in epidemic emergence or the evolution of severe fetal infections. Instead, using highly detailed phylogenetic reconstructions, Pettersson et al. discuss a series of mutations believed to have occurred in Southeast Asia in a region of the ZIKV matrix (M) and envelope (E) glycoprotein gene (domain III, believed to be responsible for interactions with cellular receptors of both vertebrate hosts and mosquito vectors). One or more of these E mutations could be responsible either for enhanced transmission by the primary epidemic mosquito vector, Aedes aegypti, or for altered human tropism that could explain fetal infection and congenital pathogenesis. One of these mutations, encoding an S139N substitution in the transmembrane M protein, has been identified as an important determinant of viral maturation for the closely related flavivirus DENV (10). Thus, all ZIKV strains associated with epidemic urban, mosquito-borne transmission; sexual transmission via semen; GBS; and congenital Zika syndrome include this M protein substitution along with D683E in the envelope glycoprotein and V763M and T777M in the transmembrane domain of E. Another substitution, M/T2634V, likely occurred after ZIKV spread from French Polynesia or another island in the South Pacific to the Americas (estimated from August 2013 to April 2014) and thus could be associated with the higher incidence of microcephaly in Brazil than in the South Pacific outbreaks (8, 11). Pettersson et al. also suggest that other contemporaneous substitutions in the NS4B and NS5 proteins involved in perturbation of the vertebrate interferon response could also be responsible for recent changes in human pathogenesis. Another recent finding, that ZIKV is significantly more thermostable than DENV, may also be involved in its epidemic spread by virtue of its ability to replicate to higher levels of viremia accompanied by fever and to resist inactivation under unfavorable conditions (12).

(ii) Stochastic introduction.In addition to the adaptive evolution hypothesis described above, a competing hypothesis is that ZIKV was simply transported stochastically to locations with large-enough naive human populations, accompanied by large urban mosquito vector populations, for an explosive outbreak. Under these epidemic conditions, formerly rare conditions were suddenly recognized when large enough numbers of infections occurred.

Prospects and means to test these hypotheses.Thorough testing of these two competing hypotheses for ZIKV emergence should be a high priority. Potentially altered roles of most viral proteins based on amino acid changes, such as those identified by Pettersson et al. (2) associated with ZIKV spread from Southeast Asia to the South Pacific and then on to the Americas, cannot be predicted with certainty. However, reverse genetic approaches are now available to assess the phenotype of each. Fortunately, cDNA-based clones for ZIKV are available to initiate these studies (13).

Mutations that may affect infection and transmission by the urban vector, A. aegypti, can easily be tested using experimental infections with artificial blood meals or viremic mice and wild-type, field-collected or low-generation-colonized mosquitoes. Even simpler experiments comparing African, Asian, and American ZIKV strains have thus far shown the African strains to be fitter for infection of A. aegypti mosquitoes (14) associated with transmission in the Americas (15), a finding incompatible with major adaptation for transmission by this vector.

However, assessing potential effects on human pathogenesis will be much more challenging due to the limited animal models available. Wild-type mice can be infected with ZIKV but develop no signs of disease and little or no viremia, limiting their value. Genetically modified mice defective for type I and/or type II interferon signaling, or wild-type mice with the interferon response suppressed by antibodies, develop more systemic infections with viremia; neurologic disease; high viral loads in the brain, spinal cord, and testes; and age-dependent mortality (16, 17). Infection of these mice early during pregnancy results in infection of the placenta and fetal brain, causing fetal disease and spontaneous abortion sometimes observed in ZIKV-infected pregnant women (18). However, these murine models are far from ideal based on their lack of an intact innate immune response and the much higher rates of central nervous system (CNS) and fetal disease than those associated with human infection, especially in the adult stages. Nonhuman primates, often the best models for human viral infections, appear to be more promising. In addition to generating viremia and viral loads in saliva, urine, and cerebrospinal fluid of rhesus macaques in the absence of overt disease (19), ZIKV infection of a pregnant pigtail macaque has produced fetal brain lesions (20).

Testing the stochastic introduction hypothesis will need to rely both on the findings of the experimental work to examine adaptive evolution and on surveillance and epidemiological studies. If Southeast Asian and potentially even African ZIKV strains have the same potential emergence and pathogenesis phenotypes, these findings would support the stochastic hypothesis: that ZIKV amplification in French Polynesia, followed by further spread via air travel into larger naive populations in the Americas, resulted in massive epidemics with the opportunity to detect rare, severe outcomes of infection. Recent reports of A. aegypti-borne epidemic ZIKV transmission in Singapore (21) and two cases of ZIKV-associated microcephaly in Thailand (22) suggest that no major phenotypic change in these properties occurred since the ZIKV spread from Asia. Prior to 2003 and even before 2013, there was a lack of attention and efficient diagnostics for ZIKV infection in Asia. Combined with the typical default clinical diagnosis in the absence of laboratory diagnostics of dengue for any arboviral infection producing a nonspecific acute febrile illness in that region, this calls into question the assumption that efficient urban transmission and CNS disease were absent from Asia in the past. Now that commercial diagnostics for ZIKV and antibody responses are becoming available, increased attention and diagnostic attempts may be detecting small outbreaks and congenital infections that have occurred all along. Also, the presence of nearly continuous, low-level endemic transmission in Asia (like that of CHIKV between the 1960s and 1996) may result in levels of herd immunity that limit the efficiency of mosquito-borne transmission and the number of maternal infections to a degree where the incidence of microcephaly above baseline levels caused by other infections can be overlooked, as initially occurred in French Polynesia despite more than 100,000 cases in 2013 to 2014 (8). Even more limited diagnostic capabilities in many parts of Africa could easily mask these same conditions. With the increased availability of ZIKV diagnostics (albeit with remaining limitations on serodiagnosis in locations where dengue is endemic), improved surveillance and epidemiological studies in Southeast Asia and Africa should be able to provide further data to more robustly test the second hypothesis that recent emergences were stochastic, relying on increased air travel and the arrival of ZIKV in regions with completely naive human populations.

Determining the mechanisms of epidemic ZIKV emergence, especially of the appearance of congenital Zika syndrome and GBS, is critical to predict future risks as well as to target surveillance and control measures in key locations. For example, if epidemic potential and adverse infection outcomes have recently evolved with spread to the South Pacific and the Americas, Asia and Africa may be at risk mainly from strains imported from these epidemic locations. Thanks to the ability of phylogenetic methods to reconstruct patterns of ZIKV evolution and individual mutations associated with spread and emergence (2), combined with reverse genetic systems (13), the adaptive evolution hypothesis for ZIKV emergence can now be tested experimentally in relatively short order. Improved animal models for congenital Zika syndrome and eventually for GBS can enhance this opportunity. Also, thanks to international attention on ZIKV leading to increased surveillance in Asia and hopefully also in Africa, the urban transmission and pathogenesis phenotypes of the African and Asian ZIKV lineages should be revealed in the coming years.

A major limitation on the latter analyses is the lack of specificity of serologic tests for past infection with ZIKV. Cross-reactions with antibodies induced by DENV, yellow fever virus, and other flaviviruses continue to limit the ability to assess herd immunity and to accurately diagnose many ZIKV infections. Improvements in serodiagnostics remain a critical need for understanding ZIKV emergence and also determining likely patterns of future spread and infection that can inform optimal control strategies.

FOOTNOTES

    • Published 10 January 2017
  • For the article discussed, see https://doi.org/10.1128/mBio.01239-16.

  • Copyright © 2017 Weaver.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license .

REFERENCES

  1. 1.↵
    1. Frieden TR,
    2. Schuchat A,
    3. Petersen LR
    . 2016. Zika virus 6 months later. JAMA316:1443–1444. doi:10.1001/jama.2016.11941.
    OpenUrlCrossRefPubMed
  2. 2.↵
    1. Pettersson JH,
    2. Eldholm V,
    3. Seligman SJ,
    4. Lundkvist Å,
    5. Falconar AK,
    6. Gaunt MW,
    7. Musso D,
    8. Nougairède A,
    9. Charrel R,
    10. Gould EA,
    11. de Lamballerie X
    . 2016. How did Zika virus emerge in the Pacific Islands and Latin America?mBio7:e01239-16. doi:10.1128/mBio.01239-16.
    OpenUrlAbstract/FREE Full Text
  3. 3.↵
    1. Weaver SC,
    2. Costa F,
    3. Garcia-Blanco MA,
    4. Ko AI,
    5. Ribeiro GS,
    6. Saade G,
    7. Shi PY,
    8. Vasilakis N
    . 2016. Zika virus: history, emergence, biology, and prospects for control. Antiviral Res130:69–80. doi:10.1016/j.antiviral.2016.03.010.
    OpenUrlCrossRefPubMed
  4. 4.↵
    1. Haddow AD,
    2. Schuh AJ,
    3. Yasuda CY,
    4. Kasper MR,
    5. Heang V,
    6. Huy R,
    7. Guzman H,
    8. Tesh RB,
    9. Weaver SC
    . 2012. Genetic characterization of Zika virus strains: geographic expansion of the Asian lineage. PLoS Negl Trop Dis6:e1477. doi:10.1371/journal.pntd.0001477.
    OpenUrlCrossRefPubMed
  5. 5.↵
    1. Lanciotti RS,
    2. Kosoy OL,
    3. Laven JJ,
    4. Velez JO,
    5. Lambert AJ,
    6. Johnson AJ,
    7. Stanfield SM,
    8. Duffy MR
    . 2008. Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007. Emerg Infect Dis14:1232–1239. doi:10.3201/eid1408.080287.
    OpenUrlCrossRefPubMedWeb of Science
  6. 6.↵
    1. Tsetsarkin KA,
    2. Chen R,
    3. Yun R,
    4. Rossi SL,
    5. Plante KS,
    6. Guerbois M,
    7. Forrester N,
    8. Perng GC,
    9. Sreekumar E,
    10. Leal G,
    11. Huang J,
    12. Mukhopadhyay S,
    13. Weaver SC
    . 2014. Multi-peaked adaptive landscape for chikungunya virus evolution predicts continued fitness optimization in Aedes albopictus mosquitoes. Nat Commun5:4084. doi:10.1038/ncomms5084.
    OpenUrlCrossRefPubMed
  7. 7.↵
    1. Oehler E,
    2. Watrin L,
    3. Larre P,
    4. Leparc-Goffart I,
    5. Lastere S,
    6. Valour F,
    7. Baudouin L,
    8. Mallet H,
    9. Musso D,
    10. Ghawche F
    . 2014. Zika virus infection complicated by Guillain-Barre syndrome—case report, French Polynesia, December 2013. Euro Surveill19:20720. doi:10.2807/1560-7917.ES2014.19.9.20720.
    OpenUrlCrossRefPubMed
  8. 8.↵
    1. Cauchemez S,
    2. Besnard M,
    3. Bompard P,
    4. Dub T,
    5. Guillemette-Artur P,
    6. Eyrolle-Guignot D,
    7. Salje H,
    8. Van Kerkhove MD,
    9. Abadie V,
    10. Garel C,
    11. Fontanet A,
    12. Mallet HP
    . 2016. Association between Zika virus and microcephaly in French Polynesia, 2013–15: a retrospective study. Lancet387:2125–2132. doi:10.1016/S0140-6736(16)00651-6.
    OpenUrlCrossRefPubMed
  9. 9.↵
    1. Zanluca C,
    2. Melo VC,
    3. Mosimann AL,
    4. Santos GI,
    5. Santos CN,
    6. Luz K
    . 2015. First report of autochthonous transmission of Zika virus in Brazil. Mem Inst Oswaldo Cruz110:569–572. doi:10.1590/0074-02760150192.
    OpenUrlCrossRefPubMed
  10. 10.↵
    1. Sirohi D,
    2. Chen Z,
    3. Sun L,
    4. Klose T,
    5. Pierson TC,
    6. Rossmann MG,
    7. Kuhn RJ
    . 2016. The 3.8 A resolution cryo-EM structure of Zika virus. Science352:467–470. doi:10.1126/science.aaf5316.
    OpenUrlAbstract/FREE Full Text
  11. 11.↵
    1. Brasil P,
    2. Pereira JP Jr.,
    3. Raja Gabaglia C,
    4. Damasceno L,
    5. Wakimoto M,
    6. Ribeiro Nogueira RM,
    7. Carvalho de Sequeira P,
    8. Machado Siqueira A,
    9. Abreu de Carvalho LM,
    10. Cotrim da Cunha D,
    11. Calvet GA,
    12. Neves ES,
    13. Moreira ME,
    14. Rodrigues Baiao AE,
    15. Nassar de Carvalho PR,
    16. Janzen C,
    17. Valderramos SG,
    18. Cherry JD,
    19. Bispo de Filippis AM,
    20. Nielsen-Saines K
    . 4 March2016. Zika virus infection in pregnant women in Rio de Janeiro—preliminary report. N Engl J Med doi:10.1056/NEJMoa1602412.
    OpenUrlCrossRefPubMed
  12. 12.↵
    1. Kostyuchenko VA,
    2. Lim EX,
    3. Zhang S,
    4. Fibriansah G,
    5. Ng TS,
    6. Ooi JS,
    7. Shi J,
    8. Lok SM
    . 2016. Structure of the thermally stable Zika virus. Nature533:425–428. doi:10.1038/nature17994.
    OpenUrlCrossRef
  13. 13.↵
    1. Shan C,
    2. Xie X,
    3. Muruato AE,
    4. Rossi SL,
    5. Roundy CM,
    6. Azar SR,
    7. Yang Y,
    8. Tesh RB,
    9. Bourne N,
    10. Barrett AD,
    11. Vasilakis N,
    12. Weaver SC,
    13. Shi PY
    . 2016. An infectious cDNA clone of Zika virus to study viral virulence, mosquito transmission, and antiviral inhibitors. Cell Host Microbe19:891–900. doi:10.1016/j.chom.2016.05.004.
    OpenUrlCrossRefPubMed
  14. 14.↵
    1. Weger-Lucarelli J,
    2. Rückert C,
    3. Chotiwan N,
    4. Nguyen C,
    5. Garcia Luna SM,
    6. Fauver JR,
    7. Foy BD,
    8. Perera R,
    9. Black WC,
    10. Kading RC,
    11. Ebel GD
    . 2016. Vector competence of American mosquitoes for three strains of Zika virus. PLoS Negl Trop Dis10:e0005101. doi:10.1371/journal.pntd.0005101.
    OpenUrlCrossRef
  15. 15.↵
    1. Guerbois M,
    2. Fernandez-Salas I,
    3. Azar SR,
    4. Danis-Lozano R,
    5. Alpuche-Aranda CM,
    6. Leal G,
    7. Garcia-Malo IR,
    8. Diaz-Gonzalez EE,
    9. Casas-Martinez M,
    10. Rossi SL,
    11. Del Río-Galván SL,
    12. Sanchez-Casas RM,
    13. Roundy CM,
    14. Wood TG,
    15. Widen SG,
    16. Vasilakis N,
    17. Weaver SC
    . 2016. Outbreak of Zika virus infection, Chiapas State, Mexico, 2015, and first confirmed transmission by Aedes aegypti mosquitoes in the Americas. J Infect Dis214:1349–1356. doi:10.1093/infdis/jiw302.
    OpenUrlCrossRefPubMed
  16. 16.↵
    1. Rossi SL,
    2. Tesh RB,
    3. Azar SR,
    4. Muruato AE,
    5. Hanley KA,
    6. Auguste AJ,
    7. Langsjoen RM,
    8. Paessler S,
    9. Vasilakis N,
    10. Weaver SC
    . 2016. Characterization of a novel murine model to study Zika virus. Am J Trop Med Hyg94:1362–1369. doi:10.4269/ajtmh.16-0111.
    OpenUrlAbstract/FREE Full Text
  17. 17.↵
    1. Lazear HM,
    2. Govero J,
    3. Smith AM,
    4. Platt DJ,
    5. Fernandez E,
    6. Miner JJ,
    7. Diamond MS
    . 2016. A mouse model of Zika virus pathogenesis. Cell Host Microbe19:720–730. doi:10.1016/j.chom.2016.03.010.
    OpenUrlCrossRefPubMed
  18. 18.↵
    1. Miner JJ,
    2. Cao B,
    3. Govero J,
    4. Smith AM,
    5. Fernandez E,
    6. Cabrera OH,
    7. Garber C,
    8. Noll M,
    9. Klein RS,
    10. Noguchi KK,
    11. Mysorekar IU,
    12. Diamond MS
    . 2016. Zika virus infection during pregnancy in mice causes placental damage and fetal demise. Cell165:1081–1091. doi:10.1016/j.cell.2016.05.008.
    OpenUrlCrossRefPubMed
  19. 19.↵
    1. Dudley DM,
    2. Aliota MT,
    3. Mohr EL,
    4. Weiler AM,
    5. Lehrer-Brey G,
    6. Weisgrau KL,
    7. Mohns MS,
    8. Breitbach ME,
    9. Rasheed MN,
    10. Newman CM,
    11. Gellerup DD,
    12. Moncla LH,
    13. Post J,
    14. Schultz-Darken N,
    15. Schotzko ML,
    16. Hayes JM,
    17. Eudailey JA,
    18. Moody MA,
    19. Permar SR,
    20. O’Connor SL,
    21. Rakasz EG,
    22. Simmons HA,
    23. Capuano S,
    24. Golos TG,
    25. Osorio JE,
    26. Friedrich TC,
    27. O’Connor DH
    . 2016. A rhesus macaque model of Asian-lineage Zika virus infection. Nat Commun7:12204. doi:10.1038/ncomms12204.
    OpenUrlCrossRefPubMed
  20. 20.↵
    1. Adams Waldorf KM,
    2. Stencel-Baerenwald JE,
    3. Kapur RP,
    4. Studholme C,
    5. Boldenow E,
    6. Vornhagen J,
    7. Baldessari A,
    8. Dighe MK,
    9. Thiel J,
    10. Merillat S,
    11. Armistead B,
    12. Tisoncik-Go J,
    13. Green RR,
    14. Davis MA,
    15. Dewey EC,
    16. Fairgrieve MR,
    17. Gatenby JC,
    18. Richards T,
    19. Garden GA,
    20. Diamond MS,
    21. Juul SE,
    22. Grant RF,
    23. Kuller L,
    24. Shaw DW,
    25. Ogle J,
    26. Gough GM,
    27. Lee W,
    28. English C,
    29. Hevner RF,
    30. Dobyns WB,
    31. Gale M Jr.,
    32. Rajagopal L
    . 2016. Fetal brain lesions after subcutaneous inoculation of Zika virus in a pregnant nonhuman primate. Nat Med22:1256–1259. doi:10.1038/nm.4193.
    OpenUrlCrossRefPubMed
  21. 21.↵
    1. Maurer-Stroh S,
    2. Mak TM,
    3. Ng YK,
    4. Phuah SP,
    5. Huber RG,
    6. Marzinek JK,
    7. Holdbrook DA,
    8. Lee RT,
    9. Cui L,
    10. Lin RT
    . 2016. South-East Asian Zika virus strain linked to cluster of cases in Singapore, August 2016. Euro Surveill21:30347. doi:10.2807/1560-7917.ES.2016.21.38.30347.
    OpenUrlCrossRef
  22. 22.↵
    1. World Health Organization
    . 6October2016. Zika situation report. World Health Organization, Geneva, Switzerland. http://www.who.int/emergencies/zika-virus/situation-report/6-october-2016/en/ .
View Abstract
PreviousNext
Back to top
Download PDF
Citation Tools
Emergence of Epidemic Zika Virus Transmission and Congenital Zika Syndrome: Are Recently Evolved Traits to Blame?
Scott C. Weaver
mBio Jan 2017, 8 (1) e02063-16; DOI: 10.1128/mBio.02063-16

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this mBio article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Emergence of Epidemic Zika Virus Transmission and Congenital Zika Syndrome: Are Recently Evolved Traits to Blame?
(Your Name) has forwarded a page to you from mBio
(Your Name) thought you would be interested in this article in mBio.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Emergence of Epidemic Zika Virus Transmission and Congenital Zika Syndrome: Are Recently Evolved Traits to Blame?
Scott C. Weaver
mBio Jan 2017, 8 (1) e02063-16; DOI: 10.1128/mBio.02063-16
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • COMMENTARY
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

epidemics
Guillain-Barre Syndrome
microcephaly
Zika Virus Infection

Related Articles

Cited By...

About

  • About mBio
  • Editor in Chief
  • Board of Editors
  • AAM Fellows
  • Policies
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Author Warranty
  • Article Types
  • Ethics
  • Contact Us

Follow #mBio

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Online ISSN: 2150-7511