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Research Article | Host-Microbe Biology

Experimental Human Challenge Defines Distinct Pneumococcal Kinetic Profiles and Mucosal Responses between Colonized and Non-Colonized Adults

Elissavet Nikolaou, Simon P. Jochems, Elena Mitsi, Sherin Pojar, Annie Blizard, Jesus Reiné, Carla Solórzano, Edessa Negera, Beatriz Carniel, Alessandra Soares-Schanoski, Victoria Connor, Hugh Adler, Seher R. Zaidi, Caz Hales, Helen Hill, Angie Hyder-Wright, Stephen B. Gordon, Jamie Rylance, Daniela M. Ferreira
N. Luisa Hiller, Editor
Elissavet Nikolaou
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
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  • ORCID record for Elissavet Nikolaou
Simon P. Jochems
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
bDepartment of Parasitology, Leiden University Medical Center, Leiden, Netherlands
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Elena Mitsi
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
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Sherin Pojar
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
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Annie Blizard
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
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Jesus Reiné
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
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Carla Solórzano
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
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Edessa Negera
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
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Beatriz Carniel
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
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Alessandra Soares-Schanoski
cBacteriology Laboratory, Butantan Institute, São Paulo, Brazil
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Victoria Connor
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
dDepartment of Respiratory Medicine, Royal Liverpool University Hospital, Liverpool, United Kingdom
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Hugh Adler
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
dDepartment of Respiratory Medicine, Royal Liverpool University Hospital, Liverpool, United Kingdom
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  • ORCID record for Hugh Adler
Seher R. Zaidi
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
dDepartment of Respiratory Medicine, Royal Liverpool University Hospital, Liverpool, United Kingdom
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Caz Hales
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
dDepartment of Respiratory Medicine, Royal Liverpool University Hospital, Liverpool, United Kingdom
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Helen Hill
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
dDepartment of Respiratory Medicine, Royal Liverpool University Hospital, Liverpool, United Kingdom
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Angie Hyder-Wright
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
dDepartment of Respiratory Medicine, Royal Liverpool University Hospital, Liverpool, United Kingdom
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Stephen B. Gordon
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
eMalawi-Liverpool Wellcome Trust Clinical Research Programme, Blantyre, Malawi
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Jamie Rylance
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
dDepartment of Respiratory Medicine, Royal Liverpool University Hospital, Liverpool, United Kingdom
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Daniela M. Ferreira
aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
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N. Luisa Hiller
Carnegie Mellon University
Roles: Editor
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DOI: 10.1128/mBio.02020-20
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Article Information

DOI 
https://doi.org/10.1128/mBio.02020-20
PubMed 
33436429

Published By 
American Society for Microbiology
History 
  • Received July 20, 2020
  • Accepted November 4, 2020
  • Published online January 12, 2021.

Copyright & Usage 
Copyright © 2021 Nikolaou et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Editors and / or Reviewers 
  • N. Luisa Hiller, Editor, Carnegie Mellon University

Author Information

  1. Elissavet Nikolaoua,
  2. Simon P. Jochemsa,b,
  3. Elena Mitsia,
  4. Sherin Pojara,
  5. Annie Blizarda,
  6. Jesus Reinéa,
  7. Carla Solórzanoa,
  8. Edessa Negeraa,
  9. Beatriz Carniela,
  10. Alessandra Soares-Schanoskic,
  11. Victoria Connora,d,
  12. Hugh Adlera,d,
  13. Seher R. Zaidia,d,
  14. Caz Halesa,d,
  15. Helen Hilla,d,
  16. Angie Hyder-Wrighta,d,
  17. Stephen B. Gordona,e,
  18. Jamie Rylancea,d,
  19. Daniela M. Ferreiraa
  1. aDepartment of Clinical Sciences, Liverpool School of Tropical Medicine, Liverpool, United Kingdom
  2. bDepartment of Parasitology, Leiden University Medical Center, Leiden, Netherlands
  3. cBacteriology Laboratory, Butantan Institute, São Paulo, Brazil
  4. dDepartment of Respiratory Medicine, Royal Liverpool University Hospital, Liverpool, United Kingdom
  5. eMalawi-Liverpool Wellcome Trust Clinical Research Programme, Blantyre, Malawi
  • Address correspondence to Elissavet Nikolaou, elissavet.nikolaou{at}lstmed.ac.uk, Simon P. Jochems, s.p.jochems{at}lumc.nl, or Daniela M. Ferreira, daniela.ferreira{at}lstmed.ac.uk.
  • Elissavet Nikolaou and Simon P. Jochems contributed equally to this work. Author order was determined by tossing a coin.

  • Citation Nikolaou E, Jochems SP, Mitsi E, Pojar S, Blizard A, Reiné J, Solórzano C, Negera E, Carniel B, Soares-Schanoski A, Connor V, Adler H, Zaidi SR, Hales C, Hill H, Hyder-Wright A, Gordon SB, Rylance J, Ferreira DM. 2021. Experimental human challenge defines distinct pneumococcal kinetic profiles and mucosal responses between colonized and non-colonized adults. mBio 12:e02020-20. https://doi.org/10.1128/mBio.02020-20.

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Experimental Human Challenge Defines Distinct Pneumococcal Kinetic Profiles and Mucosal Responses between Colonized and Non-Colonized Adults
Elissavet Nikolaou, Simon P. Jochems, Elena Mitsi, Sherin Pojar, Annie Blizard, Jesus Reiné, Carla Solórzano, Edessa Negera, Beatriz Carniel, Alessandra Soares-Schanoski, Victoria Connor, Hugh Adler, Seher R. Zaidi, Caz Hales, Helen Hill, Angie Hyder-Wright, Stephen B. Gordon, Jamie Rylance, Daniela M. Ferreira
mBio Jan 2021, 12 (1) e02020-20; DOI: 10.1128/mBio.02020-20

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Experimental Human Challenge Defines Distinct Pneumococcal Kinetic Profiles and Mucosal Responses between Colonized and Non-Colonized Adults
Elissavet Nikolaou, Simon P. Jochems, Elena Mitsi, Sherin Pojar, Annie Blizard, Jesus Reiné, Carla Solórzano, Edessa Negera, Beatriz Carniel, Alessandra Soares-Schanoski, Victoria Connor, Hugh Adler, Seher R. Zaidi, Caz Hales, Helen Hill, Angie Hyder-Wright, Stephen B. Gordon, Jamie Rylance, Daniela M. Ferreira
mBio Jan 2021, 12 (1) e02020-20; DOI: 10.1128/mBio.02020-20
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KEYWORDS

Streptococcus pneumoniae
colonization
saliva
nasal lining fluid
cytokines
neutrophil acquisition
host-pathogens interactions
controlled human infection

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