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Research Article | Host-Microbe Biology

Human Neutrophils Produce Antifungal Extracellular Vesicles against Aspergillus fumigatus

Iordana A. Shopova, Ivan Belyaev, Prasad Dasari, Susanne Jahreis, Maria C. Stroe, Zoltán Cseresnyés, Ann-Kathrin Zimmermann, Anna Medyukhina, Carl-Magnus Svensson, Thomas Krüger, Viktòria Szeifert, Sandor Nietzsche, Theresia Conrad, Matthew G. Blango, Olaf Kniemeyer, Marie von Lilienfeld-Toal, Peter F. Zipfel, Erzsébet Ligeti, Marc Thilo Figge, Axel A. Brakhage
Antonio Di Pietro, Editor
Iordana A. Shopova
aInstitute of Microbiology, Friedrich Schiller University, Jena, Germany
bDepartment of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
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Ivan Belyaev
cResearch Group Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
iFriedrich Schiller University, Jena, Germany
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Prasad Dasari
dDepartment of Infection Biology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
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Susanne Jahreis
eClinic of Internal Medicine II, Haematology and Oncology, Jena University Hospital, Jena, Germany
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Maria C. Stroe
aInstitute of Microbiology, Friedrich Schiller University, Jena, Germany
bDepartment of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
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Zoltán Cseresnyés
cResearch Group Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
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Ann-Kathrin Zimmermann
aInstitute of Microbiology, Friedrich Schiller University, Jena, Germany
bDepartment of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
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Anna Medyukhina
cResearch Group Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
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Carl-Magnus Svensson
cResearch Group Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
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Thomas Krüger
bDepartment of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
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Viktòria Szeifert
fDepartment of Physiology, Semmelweis University, Budapest, Hungary
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Sandor Nietzsche
gCentre for Electron Microscopy, Jena University Hospital, Jena, Germany
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Theresia Conrad
hResearch Group Systems Biology and Bioinformatics, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
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Matthew G. Blango
bDepartment of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
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Olaf Kniemeyer
bDepartment of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
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Marie von Lilienfeld-Toal
eClinic of Internal Medicine II, Haematology and Oncology, Jena University Hospital, Jena, Germany
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Peter F. Zipfel
aInstitute of Microbiology, Friedrich Schiller University, Jena, Germany
dDepartment of Infection Biology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
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Erzsébet Ligeti
fDepartment of Physiology, Semmelweis University, Budapest, Hungary
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Marc Thilo Figge
aInstitute of Microbiology, Friedrich Schiller University, Jena, Germany
cResearch Group Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
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Axel A. Brakhage
aInstitute of Microbiology, Friedrich Schiller University, Jena, Germany
bDepartment of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
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Antonio Di Pietro
Universidad de Córdoba
Roles: Editor
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DOI: 10.1128/mBio.00596-20
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Article Information

DOI 
https://doi.org/10.1128/mBio.00596-20
PubMed 
32291301

Published By 
American Society for Microbiology
History 
  • Received March 16, 2020
  • Accepted March 17, 2020
  • Published online April 14, 2020.

Copyright & Usage 
Copyright © 2020 Shopova et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Editors and / or Reviewers 
  • Antonio Di Pietro, Editor, Universidad de Córdoba

Author Information

  1. Iordana A. Shopovaa,b,
  2. Ivan Belyaevc,i,
  3. Prasad Dasarid,
  4. Susanne Jahreise,
  5. Maria C. Stroea,b,
  6. Zoltán Cseresnyésc,
  7. Ann-Kathrin Zimmermanna,b,
  8. Anna Medyukhinac,
  9. Carl-Magnus Svenssonc,
  10. Thomas Krügerb,
  11. Viktòria Szeifertf,
  12. Sandor Nietzscheg,
  13. Theresia Conradh,
  14. Matthew G. Blangob,
  15. Olaf Kniemeyerb,
  16. Marie von Lilienfeld-Toale,
  17. Peter F. Zipfela,d,
  18. Erzsébet Ligetif,
  19. Marc Thilo Figgea,c,
  20. Axel A. Brakhagea,b
  1. aInstitute of Microbiology, Friedrich Schiller University, Jena, Germany
  2. bDepartment of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
  3. cResearch Group Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
  4. dDepartment of Infection Biology, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
  5. eClinic of Internal Medicine II, Haematology and Oncology, Jena University Hospital, Jena, Germany
  6. fDepartment of Physiology, Semmelweis University, Budapest, Hungary
  7. gCentre for Electron Microscopy, Jena University Hospital, Jena, Germany
  8. hResearch Group Systems Biology and Bioinformatics, Leibniz Institute for Natural Product Research and Infection Biology (HKI), Jena, Germany
  9. iFriedrich Schiller University, Jena, Germany
  • Address correspondence to Axel A. Brakhage, axel.brakhage{at}uni-jena.de.
  • Citation Shopova IA, Belyaev I, Dasari P, Jahreis S, Stroe MC, Cseresnyés Z, Zimmermann A-K, Medyukhina A, Svensson C-M, Krüger T, Szeifert V, Nietzsche S, Conrad T, Blango MG, Kniemeyer O, von Lilienfeld-Toal M, Zipfel PF, Ligeti E, Figge MT, Brakhage AA. 2020. Human neutrophils produce antifungal extracellular vesicles against Aspergillus fumigatus. mBio 11:e00596-20. https://doi.org/10.1128/mBio.00596-20.

  1. This article is a direct contribution from Axel A. Brakhage, a Fellow of the American Academy of Microbiology, who arranged for and secured reviews by David Perlin, Hackensack Meridian Health Center for Discovery and Innovation, and James Kronstad, University of British Columbia.

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Human Neutrophils Produce Antifungal Extracellular Vesicles against Aspergillus fumigatus
Iordana A. Shopova, Ivan Belyaev, Prasad Dasari, Susanne Jahreis, Maria C. Stroe, Zoltán Cseresnyés, Ann-Kathrin Zimmermann, Anna Medyukhina, Carl-Magnus Svensson, Thomas Krüger, Viktòria Szeifert, Sandor Nietzsche, Theresia Conrad, Matthew G. Blango, Olaf Kniemeyer, Marie von Lilienfeld-Toal, Peter F. Zipfel, Erzsébet Ligeti, Marc Thilo Figge, Axel A. Brakhage
mBio Apr 2020, 11 (2) e00596-20; DOI: 10.1128/mBio.00596-20

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Human Neutrophils Produce Antifungal Extracellular Vesicles against Aspergillus fumigatus
Iordana A. Shopova, Ivan Belyaev, Prasad Dasari, Susanne Jahreis, Maria C. Stroe, Zoltán Cseresnyés, Ann-Kathrin Zimmermann, Anna Medyukhina, Carl-Magnus Svensson, Thomas Krüger, Viktòria Szeifert, Sandor Nietzsche, Theresia Conrad, Matthew G. Blango, Olaf Kniemeyer, Marie von Lilienfeld-Toal, Peter F. Zipfel, Erzsébet Ligeti, Marc Thilo Figge, Axel A. Brakhage
mBio Apr 2020, 11 (2) e00596-20; DOI: 10.1128/mBio.00596-20
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KEYWORDS

Aspergillus fumigatus
azurocidin
cathepsin G
extracellular vesicle
fluorescent image analysis
fungi
microvesicle
neutrophils
polymorphonuclear leukocytes

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