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Research Article | Applied and Environmental Science

Multiplex Genetic Engineering Exploiting Pyrimidine Salvage Pathway-Based Endogenous Counterselectable Markers

Lukas Birštonas, Alex Dallemulle, Manuel S. López-Berges, Ilse D. Jacobsen, Martin Offterdinger, Beate Abt, Maria Straßburger, Ingo Bauer, Oliver Schmidt, Bettina Sarg, Herbert Lindner, Hubertus Haas, Fabio Gsaller
Gustavo H. Goldman, Editor
Lukas Birštonas
aInstitute of Molecular Biology/Biocenter, Innsbruck Medical University, Innsbruck, Austria
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Alex Dallemulle
aInstitute of Molecular Biology/Biocenter, Innsbruck Medical University, Innsbruck, Austria
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Manuel S. López-Berges
aInstitute of Molecular Biology/Biocenter, Innsbruck Medical University, Innsbruck, Austria
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Ilse D. Jacobsen
bResearch Group Microbial Immunology, Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany
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Martin Offterdinger
cInstitute of Neurobiochemistry/Biocenter, Innsbruck Medical University, Innsbruck, Austria
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Beate Abt
aInstitute of Molecular Biology/Biocenter, Innsbruck Medical University, Innsbruck, Austria
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Maria Straßburger
dTransfer Group of Antiinfectives, Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany
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Ingo Bauer
aInstitute of Molecular Biology/Biocenter, Innsbruck Medical University, Innsbruck, Austria
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  • ORCID record for Ingo Bauer
Oliver Schmidt
eInstitute of Cell Biology/Biocenter, Innsbruck Medical University, Innsbruck, Austria
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Bettina Sarg
fInstitute of Clinical Biochemistry/Biocenter, Innsbruck Medical University, Innsbruck, Austria
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Herbert Lindner
fInstitute of Clinical Biochemistry/Biocenter, Innsbruck Medical University, Innsbruck, Austria
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Hubertus Haas
aInstitute of Molecular Biology/Biocenter, Innsbruck Medical University, Innsbruck, Austria
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Fabio Gsaller
aInstitute of Molecular Biology/Biocenter, Innsbruck Medical University, Innsbruck, Austria
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Gustavo H. Goldman
Universidade de São Paulo
Roles: Editor
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DOI: 10.1128/mBio.00230-20
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Article Information

DOI 
https://doi.org/10.1128/mBio.00230-20
PubMed 
32265325

Published By 
American Society for Microbiology
History 
  • Received February 5, 2020
  • Accepted March 16, 2020
  • Published online April 7, 2020.

Copyright & Usage 
Copyright © 2020 Birštonas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Editors and / or Reviewers 
  • Gustavo H. Goldman, Editor, Universidade de São Paulo

Author Information

  1. Lukas Birštonasa,
  2. Alex Dallemullea,
  3. Manuel S. López-Bergesa*,
  4. Ilse D. Jacobsenb,
  5. Martin Offterdingerc,
  6. Beate Abta,
  7. Maria Straßburgerd,
  8. Ingo Bauera,
  9. Oliver Schmidte,
  10. Bettina Sargf,
  11. Herbert Lindnerf,
  12. Hubertus Haasa,
  13. Fabio Gsallera
  1. aInstitute of Molecular Biology/Biocenter, Innsbruck Medical University, Innsbruck, Austria
  2. bResearch Group Microbial Immunology, Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany
  3. cInstitute of Neurobiochemistry/Biocenter, Innsbruck Medical University, Innsbruck, Austria
  4. dTransfer Group of Antiinfectives, Leibniz Institute for Natural Product Research and Infection Biology-Hans Knöll Institute, Jena, Germany
  5. eInstitute of Cell Biology/Biocenter, Innsbruck Medical University, Innsbruck, Austria
  6. fInstitute of Clinical Biochemistry/Biocenter, Innsbruck Medical University, Innsbruck, Austria
  • Address correspondence to Fabio Gsaller, fabio.gsaller{at}i-med.ac.at.
  • ↵* Present address: Manuel S. López-Berges, Department of Genetics, University of Córdoba, Córdoba, Spain.

  • Citation Birštonas L, Dallemulle A, López-Berges MS, Jacobsen ID, Offterdinger M, Abt B, Straßburger M, Bauer I, Schmidt O, Sarg B, Lindner H, Haas H, Gsaller F. 2020. Multiplex genetic engineering exploiting pyrimidine salvage pathway-based endogenous counterselectable markers. mBio 11:e00230-20. https://doi.org/10.1128/mBio.00230-20.

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Multiplex Genetic Engineering Exploiting Pyrimidine Salvage Pathway-Based Endogenous Counterselectable Markers
Lukas Birštonas, Alex Dallemulle, Manuel S. López-Berges, Ilse D. Jacobsen, Martin Offterdinger, Beate Abt, Maria Straßburger, Ingo Bauer, Oliver Schmidt, Bettina Sarg, Herbert Lindner, Hubertus Haas, Fabio Gsaller
mBio Apr 2020, 11 (2) e00230-20; DOI: 10.1128/mBio.00230-20

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Multiplex Genetic Engineering Exploiting Pyrimidine Salvage Pathway-Based Endogenous Counterselectable Markers
Lukas Birštonas, Alex Dallemulle, Manuel S. López-Berges, Ilse D. Jacobsen, Martin Offterdinger, Beate Abt, Maria Straßburger, Ingo Bauer, Oliver Schmidt, Bettina Sarg, Herbert Lindner, Hubertus Haas, Fabio Gsaller
mBio Apr 2020, 11 (2) e00230-20; DOI: 10.1128/mBio.00230-20
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KEYWORDS

endogenous selectable markers
genetic engineering
pyrimidine salvage pathway
targeted genomic integrations

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