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Research Article | Host-Microbe Biology

The Toxoplasma gondii Cyst Wall Interactome

Vincent Tu, Tadakimi Tomita, Tatsuki Sugi, Joshua Mayoral, Bing Han, Rama R. Yakubu, Tere Williams, Aline Horta, Yanfen Ma, Louis M. Weiss
Anita A. Koshy, Editor
Vincent Tu
aDepartment of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA
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Tadakimi Tomita
aDepartment of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA
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  • ORCID record for Tadakimi Tomita
Tatsuki Sugi
aDepartment of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA
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Joshua Mayoral
aDepartment of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA
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Bing Han
aDepartment of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA
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Rama R. Yakubu
aDepartment of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA
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Tere Williams
aDepartment of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA
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Aline Horta
aDepartment of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA
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Yanfen Ma
aDepartment of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA
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Louis M. Weiss
aDepartment of Pathology, Albert Einstein College of Medicine, Bronx, New York, USA
bDepartment of Medicine, Albert Einstein College of Medicine, Bronx, New York, USA
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Anita A. Koshy
University of Arizona
Roles: Editor
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DOI: 10.1128/mBio.02699-19
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ABSTRACT

A characteristic of the latent cyst stage of Toxoplasma gondii is a thick cyst wall that forms underneath the membrane of the bradyzoite vacuole. Previously, our laboratory group published a proteomic analysis of purified in vitro cyst wall fragments that identified an inventory of cyst wall components. To further refine our understanding of the composition of the cyst wall, several cyst wall proteins were tagged with a promiscuous biotin ligase (BirA*), and their interacting partners were screened by streptavidin affinity purification. Within the cyst wall pulldowns, previously described cyst wall proteins, dense granule proteins, and uncharacterized hypothetical proteins were identified. Several of the newly identified hypothetical proteins were validated to be novel components of the cyst wall and tagged with BirA* to expand the model of the cyst wall interactome. Community detection of the cyst wall interactome model revealed three distinct clusters: a dense granule, a cyst matrix, and a cyst wall cluster. Characterization of several of the identified cyst wall proteins using genetic strategies revealed that MCP3 affects in vivo cyst sizes. This study provides a model of the potential protein interactions within the cyst wall and the groundwork to understand cyst wall formation.

IMPORTANCE A model of the cyst wall interactome was constructed using proteins identified through BioID. The proteins within this cyst wall interactome model encompass several proteins identified in a prior characterization of the cyst wall proteome. This model provides a more comprehensive understanding of the composition of the cyst wall and may lead to insights on how the cyst wall is formed.

  • Copyright © 2020 Tu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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The Toxoplasma gondii Cyst Wall Interactome
Vincent Tu, Tadakimi Tomita, Tatsuki Sugi, Joshua Mayoral, Bing Han, Rama R. Yakubu, Tere Williams, Aline Horta, Yanfen Ma, Louis M. Weiss
mBio Feb 2020, 11 (1) e02699-19; DOI: 10.1128/mBio.02699-19

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The Toxoplasma gondii Cyst Wall Interactome
Vincent Tu, Tadakimi Tomita, Tatsuki Sugi, Joshua Mayoral, Bing Han, Rama R. Yakubu, Tere Williams, Aline Horta, Yanfen Ma, Louis M. Weiss
mBio Feb 2020, 11 (1) e02699-19; DOI: 10.1128/mBio.02699-19
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KEYWORDS

BirA
cyst wall
Toxoplasma gondii
protein interactions
proteomics

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