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Research Article | Molecular Biology and Physiology

A CRISPR Interference System for Efficient and Rapid Gene Knockdown in Caulobacter crescentus

Mathilde Guzzo, Lennice K. Castro, Christopher R. Reisch, Monica S. Guo, Michael T. Laub
Erin D. Goley, Invited Editor, Arash Komeili, Editor
Mathilde Guzzo
aDepartment of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
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  • ORCID record for Mathilde Guzzo
Lennice K. Castro
aDepartment of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
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Christopher R. Reisch
aDepartment of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
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Monica S. Guo
aDepartment of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
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Michael T. Laub
aDepartment of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
bHoward Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
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Erin D. Goley
Johns Hopkins University School of Medicine
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Arash Komeili
University of California, Berkeley
Roles: Editor
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DOI: 10.1128/mBio.02415-19
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Article Information

DOI 
https://doi.org/10.1128/mBio.02415-19
PubMed 
31937638

Published By 
American Society for Microbiology
History 
  • Received September 10, 2019
  • Accepted November 27, 2019
  • Published online January 14, 2020.

Copyright & Usage 
Copyright © 2020 Guzzo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Editors and / or Reviewers 
  • Erin D. Goley, Invited Editor, Johns Hopkins University School of Medicine
  • Arash Komeili, Editor, University of California, Berkeley

Author Information

  1. Mathilde Guzzoa,
  2. Lennice K. Castroa,
  3. Christopher R. Reischa*,
  4. Monica S. Guoa,
  5. Michael T. Lauba,b
  1. aDepartment of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
  2. bHoward Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
  • Address correspondence to Michael T. Laub, laub{at}mit.edu.
  • ↵* Present address: Christopher R. Reisch, Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida, USA.

  • Citation Guzzo M, Castro LK, Reisch CR, Guo MS, Laub MT. 2020. A CRISPR interference system for efficient and rapid gene knockdown in Caulobacter crescentus. mBio 11:e02415-19. https://doi.org/10.1128/mBio.02415-19.

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A CRISPR Interference System for Efficient and Rapid Gene Knockdown in Caulobacter crescentus
Mathilde Guzzo, Lennice K. Castro, Christopher R. Reisch, Monica S. Guo, Michael T. Laub
mBio Jan 2020, 11 (1) e02415-19; DOI: 10.1128/mBio.02415-19

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A CRISPR Interference System for Efficient and Rapid Gene Knockdown in Caulobacter crescentus
Mathilde Guzzo, Lennice K. Castro, Christopher R. Reisch, Monica S. Guo, Michael T. Laub
mBio Jan 2020, 11 (1) e02415-19; DOI: 10.1128/mBio.02415-19
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KEYWORDS

CRISPR
Cas9
Caulobacter crescentus
gene knockdown

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