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Observation | Molecular Biology and Physiology

Quantitative Visualization of Gene Expression in Mucoid and Nonmucoid Pseudomonas aeruginosa Aggregates Reveals Localized Peak Expression of Alginate in the Hypoxic Zone

Peter Jorth, Melanie A. Spero, J. Livingston, Dianne K. Newman
Matthew R. Parsek, Editor
Peter Jorth
aDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA
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Melanie A. Spero
aDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA
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J. Livingston
aDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA
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Dianne K. Newman
aDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA
bDivision of Geological and Planetary Sciences, California Institute of Technology, Pasadena, California, USA
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  • ORCID record for Dianne K. Newman
Matthew R. Parsek
University of Washington
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DOI: 10.1128/mBio.02622-19
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Article Information

DOI 
https://doi.org/10.1128/mBio.02622-19
PubMed 
31848278

Published By 
American Society for Microbiology
History 
  • Received October 3, 2019
  • Accepted November 1, 2019
  • Published online December 17, 2019.

Copyright & Usage 
Copyright © 2019 Jorth et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Editors and / or Reviewers 
  • Matthew R. Parsek, Editor, University of Washington

Author Information

  1. Peter Jortha*,
  2. Melanie A. Speroa,
  3. J. Livingstona,
  4. Dianne K. Newmana,b
  1. aDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA
  2. bDivision of Geological and Planetary Sciences, California Institute of Technology, Pasadena, California, USA
  • Address correspondence to Dianne K. Newman, dkn{at}caltech.edu.
  • ↵* Present address: Peter Jorth, Department of Pathology and Laboratory Medicine, Department of Medicine, and Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, California, USA.

  • Citation Jorth P, Spero MA, Livingston J, Newman DK. 2019. Quantitative visualization of gene expression in mucoid and nonmucoid Pseudomonas aeruginosa aggregates reveals localized peak expression of alginate in the hypoxic zone. mBio 10:e02622-19. https://doi.org/10.1128/mBio.02622-19.

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Quantitative Visualization of Gene Expression in Mucoid and Nonmucoid Pseudomonas aeruginosa Aggregates Reveals Localized Peak Expression of Alginate in the Hypoxic Zone
Peter Jorth, Melanie A. Spero, J. Livingston, Dianne K. Newman
mBio Dec 2019, 10 (6) e02622-19; DOI: 10.1128/mBio.02622-19

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Quantitative Visualization of Gene Expression in Mucoid and Nonmucoid Pseudomonas aeruginosa Aggregates Reveals Localized Peak Expression of Alginate in the Hypoxic Zone
Peter Jorth, Melanie A. Spero, J. Livingston, Dianne K. Newman
mBio Dec 2019, 10 (6) e02622-19; DOI: 10.1128/mBio.02622-19
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KEYWORDS

HCR
Pseudomonas aeruginosa
aggregate
biofilms
gene expression
in situ hybridization
microscopy

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