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Research Article | Host-Microbe Biology

The Microbiota Promotes Arterial Thrombosis in Low-Density Lipoprotein Receptor-Deficient Mice

Klytaimnistra Kiouptsi, Sven Jäckel, Giulia Pontarollo, Alexandra Grill, Marijke J. E. Kuijpers, Eivor Wilms, Christian Weber, Felix Sommer, Magdolna Nagy, Carlos Neideck, Yvonne Jansen, Stefanie Ascher, Henning Formes, Cornelia Karwot, Franziska Bayer, Bettina Kollar, Saravanan Subramaniam, Michael Molitor, Philip Wenzel, Philip Rosenstiel, Hristo Todorov, Susanne Gerber, Ulrich Walter, Kerstin Jurk, Johan W. M. Heemskerk, Emiel P. C. van der Vorst, Yvonne Döring, Christoph Reinhardt
Federico Rey, Invited Editor, Nancy A. Moran, Editor
Klytaimnistra Kiouptsi
aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
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Sven Jäckel
aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
bGerman Center for Cardiovascular Research (DZHK), Partner Site RheinMain, Mainz, Germany
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Giulia Pontarollo
aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
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Alexandra Grill
aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
bGerman Center for Cardiovascular Research (DZHK), Partner Site RheinMain, Mainz, Germany
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Marijke J. E. Kuijpers
cDepartment of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
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Eivor Wilms
aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
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Christian Weber
dInstitute of Cardiovascular Prevention, Department of Medicine, Ludwig-Maximilians-University Munich, Munich, Germany
eGerman Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, Munich, Germany
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Felix Sommer
fInstitute of Clinical Molecular Biology (IKMB), Kiel University, Kiel, Germany
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  • ORCID record for Felix Sommer
Magdolna Nagy
cDepartment of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
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Carlos Neideck
cDepartment of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
dInstitute of Cardiovascular Prevention, Department of Medicine, Ludwig-Maximilians-University Munich, Munich, Germany
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Yvonne Jansen
dInstitute of Cardiovascular Prevention, Department of Medicine, Ludwig-Maximilians-University Munich, Munich, Germany
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Stefanie Ascher
aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
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Henning Formes
aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
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Cornelia Karwot
aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
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Franziska Bayer
aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
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Bettina Kollar
aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
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Saravanan Subramaniam
aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
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Michael Molitor
aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
bGerman Center for Cardiovascular Research (DZHK), Partner Site RheinMain, Mainz, Germany
gCenter for Cardiology, Cardiology I, University Medical Center Mainz, Mainz, Germany
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Philip Wenzel
aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
bGerman Center for Cardiovascular Research (DZHK), Partner Site RheinMain, Mainz, Germany
gCenter for Cardiology, Cardiology I, University Medical Center Mainz, Mainz, Germany
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Philip Rosenstiel
fInstitute of Clinical Molecular Biology (IKMB), Kiel University, Kiel, Germany
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Hristo Todorov
hInstitute of Developmental Biology and Neurobiology, Johannes Gutenberg University of Mainz, Mainz, Germany
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Susanne Gerber
hInstitute of Developmental Biology and Neurobiology, Johannes Gutenberg University of Mainz, Mainz, Germany
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Ulrich Walter
aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
bGerman Center for Cardiovascular Research (DZHK), Partner Site RheinMain, Mainz, Germany
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Kerstin Jurk
aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
bGerman Center for Cardiovascular Research (DZHK), Partner Site RheinMain, Mainz, Germany
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Johan W. M. Heemskerk
cDepartment of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
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Emiel P. C. van der Vorst
dInstitute of Cardiovascular Prevention, Department of Medicine, Ludwig-Maximilians-University Munich, Munich, Germany
eGerman Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, Munich, Germany
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Yvonne Döring
dInstitute of Cardiovascular Prevention, Department of Medicine, Ludwig-Maximilians-University Munich, Munich, Germany
eGerman Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, Munich, Germany
iDivision of Angiology, Swiss Cardiovascular Center, Inselspital, Bern University Hospital, Bern, Switzerland
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Christoph Reinhardt
aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
bGerman Center for Cardiovascular Research (DZHK), Partner Site RheinMain, Mainz, Germany
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Federico Rey
University of Wisconsin—Madison
Roles: Invited Editor
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Nancy A. Moran
University of Texas at Austin
Roles: Editor
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DOI: 10.1128/mBio.02298-19
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Article Information

DOI 
https://doi.org/10.1128/mBio.02298-19
PubMed 
31641089

Published By 
American Society for Microbiology
History 
  • Received August 30, 2019
  • Accepted September 17, 2019
  • Published online October 22, 2019.

Copyright & Usage 
Copyright © 2019 Kiouptsi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Editors and / or Reviewers 
  • Federico Rey, Invited Editor, University of Wisconsin—Madison
  • Nancy A. Moran, Editor, University of Texas at Austin

Author Information

  1. Klytaimnistra Kiouptsia,
  2. Sven Jäckela,b,
  3. Giulia Pontarolloa,
  4. Alexandra Grilla,b,
  5. Marijke J. E. Kuijpersc,
  6. Eivor Wilmsa,
  7. Christian Weberd,e,
  8. Felix Sommerf,
  9. Magdolna Nagyc,
  10. Carlos Neideckc,d,
  11. Yvonne Jansend,
  12. Stefanie Aschera,
  13. Henning Formesa,
  14. Cornelia Karwota,
  15. Franziska Bayera,
  16. Bettina Kollara,
  17. Saravanan Subramaniama,
  18. Michael Molitora,b,g,
  19. Philip Wenzela,b,g,
  20. Philip Rosenstielf,
  21. Hristo Todorovh,
  22. Susanne Gerberh,
  23. Ulrich Waltera,b,
  24. Kerstin Jurka,b,
  25. Johan W. M. Heemskerkc,
  26. Emiel P. C. van der Vorstd,e,
  27. Yvonne Döringd,e,i,
  28. Christoph Reinhardta,b
  1. aCenter for Thrombosis and Hemostasis (CTH), University Medical Center Mainz, Mainz, Germany
  2. bGerman Center for Cardiovascular Research (DZHK), Partner Site RheinMain, Mainz, Germany
  3. cDepartment of Biochemistry, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands
  4. dInstitute of Cardiovascular Prevention, Department of Medicine, Ludwig-Maximilians-University Munich, Munich, Germany
  5. eGerman Center for Cardiovascular Research (DZHK), Partner Site Munich Heart Alliance, Munich, Germany
  6. fInstitute of Clinical Molecular Biology (IKMB), Kiel University, Kiel, Germany
  7. gCenter for Cardiology, Cardiology I, University Medical Center Mainz, Mainz, Germany
  8. hInstitute of Developmental Biology and Neurobiology, Johannes Gutenberg University of Mainz, Mainz, Germany
  9. iDivision of Angiology, Swiss Cardiovascular Center, Inselspital, Bern University Hospital, Bern, Switzerland
  • Address correspondence to Christoph Reinhardt, Christoph.Reinhardt{at}unimedizin-mainz.de.
  • K.K., S.J., and G.P. are co-first authors. E.P.C.V.D.V., Y.D., and C.R. are co-senior authors.

  • Citation Kiouptsi K, Jäckel S, Pontarollo G, Grill A, Kuijpers MJE, Wilms E, Weber C, Sommer F, Nagy M, Neideck C, Jansen Y, Ascher S, Formes H, Karwot C, Bayer F, Kollar B, Subramaniam S, Molitor M, Wenzel P, Rosenstiel P, Todorov H, Gerber S, Walter U, Jurk K, Heemskerk JWM, van der Vorst EPC, Döring Y, Reinhardt C. 2019. The microbiota promotes arterial thrombosis in low-density lipoprotein receptor-deficient mice. mBio 10:e02298-19. https://doi.org/10.1128/mBio.02298-19.

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The Microbiota Promotes Arterial Thrombosis in Low-Density Lipoprotein Receptor-Deficient Mice
Klytaimnistra Kiouptsi, Sven Jäckel, Giulia Pontarollo, Alexandra Grill, Marijke J. E. Kuijpers, Eivor Wilms, Christian Weber, Felix Sommer, Magdolna Nagy, Carlos Neideck, Yvonne Jansen, Stefanie Ascher, Henning Formes, Cornelia Karwot, Franziska Bayer, Bettina Kollar, Saravanan Subramaniam, Michael Molitor, Philip Wenzel, Philip Rosenstiel, Hristo Todorov, Susanne Gerber, Ulrich Walter, Kerstin Jurk, Johan W. M. Heemskerk, Emiel P. C. van der Vorst, Yvonne Döring, Christoph Reinhardt
mBio Oct 2019, 10 (5) e02298-19; DOI: 10.1128/mBio.02298-19

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The Microbiota Promotes Arterial Thrombosis in Low-Density Lipoprotein Receptor-Deficient Mice
Klytaimnistra Kiouptsi, Sven Jäckel, Giulia Pontarollo, Alexandra Grill, Marijke J. E. Kuijpers, Eivor Wilms, Christian Weber, Felix Sommer, Magdolna Nagy, Carlos Neideck, Yvonne Jansen, Stefanie Ascher, Henning Formes, Cornelia Karwot, Franziska Bayer, Bettina Kollar, Saravanan Subramaniam, Michael Molitor, Philip Wenzel, Philip Rosenstiel, Hristo Todorov, Susanne Gerber, Ulrich Walter, Kerstin Jurk, Johan W. M. Heemskerk, Emiel P. C. van der Vorst, Yvonne Döring, Christoph Reinhardt
mBio Oct 2019, 10 (5) e02298-19; DOI: 10.1128/mBio.02298-19
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KEYWORDS

gut microbiota
germfree
low-density lipoprotein receptor
arterial thrombosis
atherothrombosis
carotid artery
atherosclerosis
microbiota
platelets
vascular inflammation

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