Massively Parallel Fitness Profiling Reveals Multiple Novel Enzymes in Pseudomonas putida Lysine Metabolism

DOI: 10.1128/mBio.02577-18

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This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.

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^aJoint BioEnergy Institute, Emeryville, California, USA
^bBiological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA
^cDepartment of Plant and Microbial Biology, University of California, Berkeley, Berkeley, California, USA
^dDepartment of Chemistry, University of California, Berkeley, Berkeley, California, USA
^eCentro de Biotecnología FEMSA, Tecnológico de Monterrey, Monterrey, NL, Mexico
^fDepartment of Energy Agile BioFoundry, Emeryville, California, USA
^gComparative Biochemistry Graduate Group, University of California, Berkeley, Berkeley, California, USA
^hDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, California, USA
ⁱMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA
^jJoint Program in Bioengineering, University of California, Berkeley, Berkeley, California, USA
^kEnvironmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA
^lThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark

Address correspondence to Jay D. Keasling, jdkeasling{at}lbl.gov.
Citation Thompson MG, Blake-Hedges JM, Cruz-Morales P, Barajas JF, Curran SC, Eiben CB, Harris NC, Benites VT, Gin JW, Sharpless WA, Twigg FF, Skyrud W, Krishna RN, Henrique Pereira J, Baidoo EEK, Petzold CJ, Adams PD, Arkin AP, Deutschbauer AM, Keasling JD. 2019. Massively parallel fitness profiling reveals multiple novel enzymes in Pseudomonas putida lysine metabolism. mBio 10:e02577-18. https://doi.org/10.1128/mBio.02577-18.

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